Non-Radiologic Assessment of Kidney Stones

oxfordgenomesciences

Non-Radiologic Assessment of Kidney Stones by KIT, a Spot Urine Assay.

 To evaluate the utility of the KIT Assay urinary biomarkers to detect kidney stones and quantify stone burden.</AbstractText><AbstractText>136 spot urine samples from 98 individuals, with and without kidney stone disease, were processed for measuring a pre-defined assay consisting of 6 DNA and protein markers, to generate a risk score for non-invasive detection of nephrolithiasis. From this cohort, 56 individuals had spot, non-timed, urine samples collected at the time of radiographically confirmed kidney stones and 54 demographically matched, healthy controls, without kidney stone disease, also provided spot, non-timed urine samples. Sixteen individuals with persistent stone disease had more than one urine sample.

Using a proprietary microwell based kidney injury test (KIT) assay, we measured cell-free DNA, methylated cell-free DNA, clusterin, creatinine, protein, and CXCL10. A KIT Stone-Score was computed across all markers, using the prior locked KIT algorithm. The KIT Stone-Score, scaled from 0 – 100, was then correlated with demographic variables, kidney stone burden, obstructive kidney stone disease, and urine solutes by 24-hour urine collections.

The scaled KIT stone-score (KITstone), as a composite of all 6 biomarkers, readily discriminated individuals with current or prior radiographically confirmed kidney stones, from healthy controls without kidney stone disease (P < 0.0001). KITstone also correlated in individuals with nephrolithiasis, with radiologically measured stone size (P = 0.0174) and also differentiated patients with a clinical radiological diagnosis of obstructive nephrolithiasis, associated with upper renal tract dilatation (P = 0.0010).

On the other hand, stone burden as assessed by KITstone, did not correlate with the any of the traditional measures of 24-hour urine solutes or the 24-hour urine supersaturation levels. In patients with persistent stone disease, where multiple urine samples were collected over time and different interventions, KITstone could non-invasively track stone burden over time by a spot urine, non-timed urine sample.

A random, spot urine-based assay, KITstone, can non-invasively detect, quantify, and monitor current stone burden, and may thus minimize radiographic exposure for kidney stone detection. The KITstone assay may also help monitor for stone recurrence risk for patients with nephrolithiasis, without the requirement of 24 hour urine collections.

oxfordgenomesciences
oxfordgenomesciences

General Blocking Buffer-BB1

KF17337 500 ml
EUR 277.2

TBS blocking buffer with BSA

SD8113 1pk, 1L
EUR 100.72

PBS Blocking buffer with BSA

SB0626 1pk, 1L
EUR 100.72

SynBlock Blocking Buffer-BB3

KF17339 500 ml
EUR 292.8

ELISA Plate Blocking Buffer concentrate (10X) BSA-based

80060 50 ml
EUR 242.4

3 Blocking Buffer Sample Pack

KF17340 3 x 100 ml
EUR 246

EZBlock? T20 (TBS) Blocking Buffer

2140-1000 each
EUR 261.6

EZBlock? T20 (TBS) Blocking Buffer

2140-200 each
EUR 144

EZBlock? T20 (PBS) Blocking Buffer

2143-1000 each
EUR 261.6

EZBlock? T20 (PBS) Blocking Buffer

2143-200 each
EUR 144

10 x Universal Blocking Buffer

GR103005 200 mL
EUR 129

TrueBlack WB Blocking Buffer Kit

23013 1kit
EUR 416.4
Description: Minimum order quantity: 1 unit of 1kit

TrueBlack WB Blocking Buffer Kit

23013-T 1kit
EUR 150
Description: Minimum order quantity: 1 unit of 1kit

Blocking Buffer for ICC and IHC

SF40011 10 ml
EUR 153.6

Claudin Domain Containing 1 Blocking Peptide

33R-9186 100 ug
EUR 216
Description: A synthetic peptide for use as a blocking control in assays to test for specificity of CLDND1 antibody, catalog no. 70R-6340

Corning 3D Clear Blocking Buffer - 100mL

5737 EACH
EUR 325.2

ChonBlock Blocking/Sample Dilution ELISA Buffer, 100 ml

9068 100 ml
EUR 155.7
Description: ChonBlock Blocking/Sample Dilution ELISA Buffer

West-Ezier Super Blocking Buffer

W3700-010 100ml
EUR 116.4

West-Ezier Super Blocking Buffer

W3700-050 500ml
EUR 198

West-Ezier Super Blocking Buffer

W3700-100 1L
EUR 295.2

Block Wash Buffer

I044 1000 ml
EUR 632.4
Description: Block Wash Buffer by Cygnus Technologies is available in Europe via Gentaur.

T-Pro Fast Blocking Buffer (in PBS)

JK92-W001 500ml/BT
EUR 224.4

T-Pro Fast Blocking Buffer (in TBS)

JK92-W002 500ml/BT
EUR 224.4

Blocking Buffer and Coating Stabilizer

abx090811-1000ml 1000 ml
EUR 627.6

Corning 3D Clear Blocking Buffer - 30mL

5736 EACH
EUR 120

PBS Blocking buffer with non fat milk

SB0625 1pk, 1L
EUR 80.88

Casein-based PBS Blocking Buffer Pack

AR0182 500mL/Pack
EUR 84

Casein-based TBS Blocking Buffer Pack

AR0183 500mL/Pack
EUR 84

Cyclin N-Terminal Domain Containing 1 Blocking Peptide

33R-7660 100 ug
EUR 216
Description: A synthetic peptide for use as a blocking control in assays to test for specificity of CNTD1 antibody, catalog no. 70R-4549

EZBlock? Blocking Buffer and Signal Enhancer

M4104-100 each
EUR 183.6

EZBlock? Blocking Buffer and Signal Enhancer

M4104-500 each
EUR 430.8

Blocking Buffer for Lateral Flow (PBS based)

abx090808-500ml 500 ml
EUR 460.8

Blocking Buffer 3 (Multiple PARP Assay Kits)

79743 25 ml x 2
EUR 105
Description: This Blocking Buffer 3 is optimized for minimizing background in multiple PARP BPS Bioscience Assay Kits. _x000D__x000D_

T-Pro Protein Free Blocking Buffer (in PBS)

JK92-W003 500ml/BT
EUR 224.4

T-Pro Protein Free Blocking Buffer (in TBS)

JK92-W004 500ml/BT
EUR 224.4

Blocking Buffer 2 (BPS Immunotherapy Assay Kits)

79728 50 ml
EUR 110

Blocking Buffer 1 (Various Epigenetics Assay Kits)

79556 50 ml
EUR 100
Description: This Blocking Buffer 1 is optimized for minimizing background in various BPS Bioscience assay kits. The BPS Bioscience Blocking Buffer improves S/B ratio of HMTs NSD2 and SetDB2 ELISAs with respect to competitor blocking buffer by both decreasing background (0% Activity control, no enzyme) and increasing assay signal (100% Activity control, enzyme without inhibitor).

Blocking Buffer 4 (Multiple Epigenetics Assay Kits)

52100 50 ml
EUR 95
Description: This Blocking Buffer 4 is optimized for minimizing background in various chemiluminescent methyltransferase, demethylase, and TET assays.

Corning 3D Clear Starter Kit - Includes Reagent/Antibody Buffer/Blocking Buffer/Penetration Buffer/Washing Buffers

5730 EACH
EUR 955.2

West-Ez Blocking Buffer, 1 % Casein for Western Blot

W3720-050 500ml
EUR 144

West-Ez Blocking Buffer, 1 % Casein for Western Blot

W3720-100 2x500ml
EUR 176.4

West-Ez Blocking Buffer, 3 % IgG Free-BSA in TBST

W3710-010 100ml
EUR 111.6

West-Ez Blocking Buffer, 3 % IgG Free-BSA in TBST

W3710-050 500ml
EUR 165.6

West-Ez Blocking Buffer, 3 % IgG Free-BSA in TBST

W3710-100 1L
EUR 218.4

Human WW and C2 domain containing 1 (WWC1) Control/blocking peptide

AB-23075-P 100ug
EUR 196.8

Human WW and C2 domain containing 1 (WWC1) Control/blocking peptide

AB-23076-P 100ug
EUR 196.8

West-Ezier Super Blocking Buffer for Phospho Antibody

W3701-010 100ml
EUR 157.2

West-Ezier Super Blocking Buffer for Phospho Antibody

W3701-050 500ml
EUR 225.6

West-Ezier Super Blocking Buffer for Phospho Antibody

W3701-100 1L
EUR 336

West-Ez Blocking Buffer, 3 % IgG & Fat Free-BSA in TBST

W3711-010 100ml
EUR 116.4

West-Ez Blocking Buffer, 3 % IgG & Fat Free-BSA in TBST

W3711-050 500ml
EUR 174

West-Ez Blocking Buffer, 3 % IgG & Fat Free-BSA in TBST

W3711-100 1L
EUR 230.4

Mouse zinc finger, BED domain containing 3 (ZBED3) Control/blocking peptide

AB-23250-P 100ug
EUR 196.8

West-Ezier Rapid Blocking Buffer, Ready-to-use, 5 Min

W3871-050 500ml
EUR 230.4

Neptune 1000ul barrier tip

BT1000.96 8 trays of 96 / pack
EUR 70.8
Description: Low retention, Racked, Pre-Sterile, Sample Saving Surface

Mouse NLR family, pyrin domain containing 4F (NLRP4F) Control/blocking peptide

AB-23251-P 100ug
EUR 196.8

Neptune 300ul barrier tip

BT300 10 trays of 96 / pack
EUR 70.8
Description: Low retention, Racked, Pre-Sterile, Sample Saving Surface

Neptune 100ul barrier tip

BT100 10 trays of 96 / pack
EUR 70.8
Description: Low retention, Racked, Pre-Sterile, Sample Saving Surface

Neptune 200ul barrier tip

BT200 10 trays of 96 / pack
EUR 70.8
Description: Low retention, Racked, Pre-Sterile, Sample Saving Surface

Neptune 1000-1250ul barrier tip

BT1250 8 trays of 96 / pack
EUR 70.8
Description: Extended Length, Low retention, Racked, Pre-Sterile, Sample Saving Surface

Neptune 10ul barrier tip

BT10 10 trays of 96 / pack
EUR 70.8
Description: Low retention, Racked, Pre-Sterile, Sample Saving Surface

Neptune 20ul barrier tip

BT20 10 trays of 96 / pack
EUR 70.8
Description: Low retention, Racked, Pre-Sterile, Sample Saving Surface

Filter tip NEPTUNE BT10E

DD713002 PK960
EUR 116.28

Neptune Assay Diluent-AD3

KF17349 500 ml
EUR 292.8

Neptune Sample Diluent-SD3

KF17354 500 ml
EUR 292.8

Human Pleckstrin homology domain containing, family A member 7 (PLEKHA7) Control/blocking peptide

AB-23061-P 100ug
EUR 196.8

Antigen blocking and stabilizing buffer for ELISA plates (non-bovine protein formulation) (for 10-20 plates)

80065 100 ml
EUR 226.8

Purite Neptune Ultimate Annual Consumable Pack

WAT1104 EACH
EUR 1813.2

Neptune 10ul extended length barrier tip

BT10XLS3 10 trays of 96 / pack
EUR 70.8
Description: Low retention, Racked, Pre-Sterile, Sample Saving Surface

Graduated tip 300 uL fine end bulk NEPTUNE

DD713042 PK1000
EUR 21.09

Buffer Saver Blocks for MSMAXI saves 450ml of buffer

MSMAXIBSB EACH
EUR 104.4

Buffer Saver Blocks for MSCHOICE saves 190ml of buffer

MSCHOICEBSB EACH
EUR 91.2

Graduated tip 1000uL fine tip in refill ESP NEPTUNE

DD712160B PK960
EUR 38.4

Neptune Barrier tip 10ul Extended Length Filter Tip

BT10XL 10 trays of 96 / pack
EUR 70.8
Description: 10ul extended length barrier tip. Racked, Pre-Sterile, Natural Polypropylene.

buffer saving blocks pk/2

EHS1100-BUFSAVE ea
EUR 67.2

buffer saving blocks pk/2

EHS1200-BUFSAVE ea
EUR 78

buffer saving blocks pk/2

EHS1400-BUFSAVE ea
EUR 108

buffer saving blocks pk/2

EHS1500-BUFSAVE ea
EUR 118.8

A kit containing all affinity purification buffers for 10-20 antibody purifications

110300-AF 1 kit
EUR 343.2

Contactin 2 Blocking Peptide

20-abx063329
  • EUR 326.40
  • EUR 493.20
  • 1 mg
  • 5 mg

Contactin 3 Blocking Peptide

20-abx063045
  • EUR 326.40
  • EUR 493.20
  • 1 mg
  • 5 mg

Rat Nephrin Control/blocking peptide #1

NPHN11-P 100 ug
EUR 196.8

Mouse Neprilysin (NEP) Control/blocking peptide

NEP11-P 100 ug
EUR 196.8

Real Time PCR mastermix 2x, (No ROX), 100RXNs, optimized ready-to-use mastermix containing unique reaction buffer, dNTP, Hot-Start Taq DNA polymerase, and QPCR dye

ACT-RT400RF each
EUR 261.6

Mouse Lipin-1 Control/blocking peptide control/blocking peptide #1

LPN11-P 100 ug
EUR 196.8

Mouse Lipin-2 Control/blocking peptide control/blocking peptide #1

LPN21-P 100 ug
EUR 196.8

Mouse Lipin-3 Control/blocking peptide control/blocking peptide #1

LPN31-P 100 ug
EUR 196.8

Real-Time PCR Mastermix 2x, Regular Level of Rox, 100RXNs, optimized ready-to-use mastermix containing unique reaction buffer, dNTP, Hot-Start Taq DNA polymerase, and QPCR dye

ACT-RT400 each
EUR 261.6

Real-Time PCR Mastermix 2x, Low Level of Rox , 100RXNs, optimized ready-to-use mastermix containing unique reaction buffer, dNTP, Hot-Start Taq DNA polymerase, and QPCR dye

ACT-RT400LR each
EUR 261.6

Nephrin ? Blocking Peptide

AF7951-BP 1mg
EUR 234

Comparison of Commercial ELISA Kits, a Prototype Multiplex Electrochemoluminescent Assay, and a Multiplex Bead-Based Immunoassay for Detecting a Urine-Based Bladder-Cancer-Associated Diagnostic Signature.

 

The ability to accurately measure multiple proteins simultaneously in a single assay has the potential to markedly improve the efficiency of clinical tests composed of multiple biomarkers. We investigated the diagnostic accuracy of the two multiplex protein array platforms for detecting a bladder-cancer-associated diagnostic signature in samples from a cohort of 80 subjects (40 with bladder cancer). Banked urine samples collected from Kyoto and Nara Universities were compared to histologically determined bladder cancer. The concentrations of the 10 proteins (A1AT; apolipoprotein E-APOE; angiogenin-ANG; carbonic anhydrase 9-CA9; interleukin 8-IL-8; matrix metalloproteinase 9-MMP-9; matrix metalloproteinase 10-MMP10; plasminogen activator inhibitor 1-PAI-1; syndecan-SDC1; and vascular endothelial growth factor-VEGF) were monitored using two prototype multiplex array platforms and an enzyme-linked immunosorbent assay (ELISA) according to the manufacturer’s technical specifications.

The range for detecting each biomarker was improved in the multiplex assays, even though the lower limit of quantification (LLOQ) was typically lower in the commercial ELISA kits. The area under the receiver operating characteristics (AUROC) of the prototype multiplex assays was reported to be 0.97 for the multiplex bead-based immunoassay (MBA) and 0.86 for the multiplex electrochemoluminescent assay (MEA). The sensitivities and specificities for MBA were 0.93 and 0.95, respectively, and for MEA were 0.85 and 0.80, respectively. Accuracy, positive predictive values (PPV), and negative predictive values (NPV) for MBA were 0.94, 0.95, and 0.93, respectively, and for MEA were 0.83, 0.81, and 0.84, respectively. Based on these encouraging preliminary data, we believe that a multiplex protein array is a viable platform that can be utilized as an efficient and highly accurate tool to quantitate multiple proteins within biologic specimens.

A nanocellulose-based colorimetric assay kit for smartphone sensing of iron and iron-chelating deferoxamine drug in biofluids. 

The current work describes the development of a “nanopaper-based analytical device (NAD)”, through the embedding of curcumin in transparent bacterial cellulose (BC) nanopaper, as a colorimetric assay kit for monitoring of iron and deferoxamine (DFO) as iron-chelating drug in biological fluids such as serum blood, urine and saliva. The iron sensing strategy using the developed assay kit is based on the decrease of the absorbance/color intensity of curcumin-embedded in BC nanopaper (CEBC) in the presence of Fe(III), due to the formation of Fe(III)-curcumin complex. On the other hand, releasing of Fe(III) from Fe(III)-CEBC upon addition of DFO as an iron-chelating drug, due to the high affinity of this drug to Fe(III) in competition with curcumin, which leads to recovery of the decreased absorption/color intensity of Fe(III)-CEBC, is utilized for selective colorimetric monitoring of this drug.

The absorption/color changes of the fabricated assay kit as output signal can be monitored by smartphone camera or by using a spectrophotometer. The results of our developed sensor agreed well with the results from a clinical reference method for determination of Fe(III) concentration in human serum blood samples, which revealed the clinical applicability of our developed assay kit. Taken together, regarding the advantageous features of the developed sensor as an easy-to-use, non-toxic, disposable, cost-effective and portable assay kit, along with those of smartphone-based sensing, it is anticipated that this sensing bioplatform, which we name lab-on-nanopaper, will find utility for sensitive, selective and easy diagnosis of iron-related diseases (iron deficiency and iron overload) and therapeutic drug monitoring (TDM) of iron-chelating drugs in clinical analysis as well.

GABA enzymatic assay kit. 

We developed an enzymatic assay system enabling easy quantification of 4-aminobutyric acid (GABA). The reaction of GABA aminotransferase obtained from Streptomyces decoyicus NBRC 13977 was combined to those of the previously developed glutamate assay system using glutamate oxidase and peroxidase. The three-enzyme system allowing GABA-dependent dye formation due to the oxidative coupling between 4-aminoantipyrine and Trinder’s reagent enabled accurate quantification of 0.2 – 150 mg/L GABA.

A pretreatment mixture consisting of glutamate oxidase, ascorbate oxidase and catalase eliminating glutamate, ascorbate, and hydrogen peroxide, respectively, was also prepared to remove those inhibitory substances from samples. Thus, constructed assay kit was used to measure the GABA content in tomato samples. The results were almost the same as that obtained by the conventional method using liquid chromatography-tandem mass spectrometry. The kit will become a promising tool especially for the on-site measurement of GABA content in agricultural products.

Leave a Reply

Your email address will not be published. Required fields are marked *