Comparison of an Immunochromatographic Assay Kit

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Comparison of an Immunochromatographic Assay Kit with DAS-ELISA for Large-Scale Diagnosis and Molecular Discrimination of Satsuma Dwarf Virus Collected from Citrus Orchards 

Satsuma dwarf virus (SDV) seriously damages citrus production by reducing the quality and yield of fruit. To avoid contamination with SDV, mother trees are checked to be SDV-free in advance of nursery tree distribution. In this study, we compared an immunochromatographic assay (ICA) kit with double-antibody sandwich enzyme-linked immunosorbent assay (DASELISA) for large-scale diagnosis of SDV in orchardgrown trees in Shizuoka Prefecture, Japan. The two methods gave conflicting results for 11 of 1,705 samples, all of which were negative by DAS-ELISA but positive by ICA.

The samples scored as positive by either DASELISA or ICA were analyzed by reverse transcription polymerase chain reaction and all were confirmed to be positive. These results validate the use of ICA as a screening method for large-scale diagnosis. Strain discrimination revealed that 16 of 22 isolates belonged to SDV, while citrus mosaic virus (CiMV) infection only and co-infection (SDV and CiMV) were in a minority.

oxfordgenomesciences
oxfordgenomesciences
FAM FLICA CASPASE-3/7 KIT (OKSA11271)
OKSA11271 100 Tests
EUR 1524
Description: Description of target: The FAM FLICA™ Caspase 3 & 7 kit uses a quick and easy method to analyze active caspases in apoptotic cells.;Species reactivity: ;Application: FC, IF;Assay info: ;Sensitivity:
Caspase 6 Assay Kit
20-abx299018
  • EUR 1302.00
  • EUR 582.00
  • 100 tests
  • 25 tests
  • Shipped within 1 week.
FAM FLICAâ„¢ CASPASE-6 KIT (OKSA11272)
OKSA11272 25 Tests
EUR 598
Description: Description of target: FAM FLICAâ„¢ CASPASE-6 KIT;Species reactivity: ;Application: FC, IF;Assay info: ;Sensitivity:
FAM FLICAâ„¢ CASPASE-6 KIT (OKSA11273)
OKSA11273 100 Tests
EUR 1550
Description: Description of target: FAM FLICAâ„¢ CASPASE-6 KIT;Species reactivity: ;Application: FC, IF;Assay info: ;Sensitivity:
Caspase 6 Fluorometric Assay Kit
55R-1276 25 assays
EUR 287
Description: Assay Kit for detection of Capase 6 activity in the research laboratory
Caspase 6 Colorimetric Assay Kit
55R-1277 25 assays
EUR 284
Description: Assay Kit for detection of Capase 6 activity in the research laboratory
Caspase 6 Assay Kit, green
KF17204 25 Tests
EUR 304
Caspase-6 Fluorometric Assay Kit
K2014-100 100 assays
EUR 474
Caspase-6 Fluorometric Assay Kit
K2014-200 200 assays
EUR 696
Caspase-6 Fluorometric Assay Kit
K2014-25 25 assays
EUR 238
Caspase-6 Fluorometric Assay Kit
K2014-400 400 assays
EUR 1086
Caspase-6 Colorimetric Assay Kit
K2015-100 100 assays
EUR 487
Caspase-6 Colorimetric Assay Kit
K2015-200 200 assays
EUR 696
Caspase-6 Colorimetric Assay Kit
K2015-25 25 assays
EUR 238
Caspase-6 Colorimetric Assay Kit
K2015-400 400 assays
EUR 1101
Caspase-6 Fluorometric Assay Kit
K114-100
EUR 430
Caspase-6 Fluorometric Assay Kit
K114-200
EUR 615
Caspase-6 Fluorometric Assay Kit
K114-25
EUR 202
Caspase-6 Fluorometric Assay Kit
K114-400
EUR 958
Caspase-6 Colorimetric Assay Kit
K115-100
EUR 452
Caspase-6 Colorimetric Assay Kit
K115-200
EUR 615
Caspase-6 Colorimetric Assay Kit
K115-25
EUR 202
Caspase-6 Colorimetric Assay Kit
K115-400
EUR 958
6-FAM
HY-66021 500mg
EUR 312
Caspase 6 Colorimetric Cell-Based ELISA Kit
EKC1087 100ul
EUR 572
6-FAM Azide
955 100 mg
EUR 702
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12171501
6-FAM Alkyne
956 100 mg
EUR 702
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12171501
6 FAM SE
A8771-10 10 mg
EUR 174
Description: 6-FAM SE, also known as 6-Carboxyfluorescein N-hydroxysuccinimide ester or 6-Carboxyfluorescein N-succinimidyl ester, is an isomer of carboxyfluorescein and amine-reactive succinimidyl ester carboxyfluorescein.
6 FAM SE
A8771-100 100 mg
EUR 731
Description: 6-FAM SE, also known as 6-Carboxyfluorescein N-hydroxysuccinimide ester or 6-Carboxyfluorescein N-succinimidyl ester, is an isomer of carboxyfluorescein and amine-reactive succinimidyl ester carboxyfluorescein.
6 FAM SE
A8771-5.1 10 mM (in 1mL DMSO)
EUR 199
Description: 6-FAM SE, also known as 6-Carboxyfluorescein N-hydroxysuccinimide ester or 6-Carboxyfluorescein N-succinimidyl ester, is an isomer of carboxyfluorescein and amine-reactive succinimidyl ester carboxyfluorescein.
6 FAM SE
A8771-50 50 mg
EUR 453
Description: 6-FAM SE, also known as 6-Carboxyfluorescein N-hydroxysuccinimide ester or 6-Carboxyfluorescein N-succinimidyl ester, is an isomer of carboxyfluorescein and amine-reactive succinimidyl ester carboxyfluorescein.
6-FAM SE
HY-15939 100mg
EUR 612
FAM FLICAâ„¢ POLY CASPASE KIT (OKSA11268)
OKSA11268 25 Tests
EUR 591
Description: Description of target: FAM FLICAâ„¢ POLY CASPASE KIT;Species reactivity: ;Application: FC, IF;Assay info: ;Sensitivity:
FAM FLICAâ„¢ POLY CASPASE KIT (OKSA11269)
OKSA11269 100 Tests
EUR 1524
Description: Description of target: FAM FLICAâ„¢ POLY CASPASE KIT;Species reactivity: ;Application: FC, IF;Assay info: ;Sensitivity:
FAM FLICAâ„¢ CASPASE-8 KIT (OKSA11276)
OKSA11276 25 Tests
EUR 591
Description: Description of target: FAM FLICAâ„¢ CASPASE-8 KIT;Species reactivity: ;Application: FC, IF;Assay info: ;Sensitivity:
FAM FLICAâ„¢ CASPASE-8 KIT (OKSA11277)
OKSA11277 100 Tests
EUR 1524
Description: Description of target: FAM FLICAâ„¢ CASPASE-8 KIT;Species reactivity: ;Application: FC, IF;Assay info: ;Sensitivity:
FAM FLICAâ„¢ CASPASE-9 KIT (OKSA11278)
OKSA11278 25 Tests
EUR 591
Description: Description of target: FAM FLICAâ„¢ CASPASE-9 KIT;Species reactivity: ;Application: FC, IF;Assay info: ;Sensitivity:
FAM FLICAâ„¢ CASPASE-9 KIT (OKSA11279)
OKSA11279 100 Tests
EUR 1524
Description: Description of target: FAM FLICAâ„¢ CASPASE-9 KIT;Species reactivity: ;Application: FC, IF;Assay info: ;Sensitivity:
FAM FLICAâ„¢ CASPASE-2 KIT (OKSA11282)
OKSA11282 25 Tests
EUR 557
Description: Description of target: FAM FLICAâ„¢ CASPASE-2 KIT;Species reactivity: ;Application: FC, IF;Assay info: ;Sensitivity:
Caspase 6 Colorimetric Cell-Based ELISA Kit (OKAG00581)
OKAG00581 96 Wells
EUR 596
Description: Description of target: ;Species reactivity: Human, Mouse, Rat;Application: ELISA;Assay info: Assay Type: Cell-Based
Subtype: None
Detection Method: Colorimetric 450 nm;Sensitivity:
FAM-Phe-CMK Serine Protease Assay Kit
abx299010-25tests 25 tests
EUR 566
  • Shipped within 1 week.
FAM-Leu-CMK Serine Protease Assay Kit
abx299011-25tests 25 tests
EUR 566
  • Shipped within 1 week.
FAM-Leu-DAP Serine Protease Assay Kit
abx299012-25tests 25 tests
EUR 566
  • Shipped within 1 week.
FAM-Phe-DAP Serine Protease Assay Kit
abx299013-25tests 25 tests
EUR 582
  • Shipped within 1 week.
FAM-Phe-CMK FLISP™ Assay Kit
KF17314 25 Tests
EUR 291
FAM-Phe-CMK FLISP™ Assay Kit
KF17315 100 Tests
EUR 695
FAM-Lue-CMK FLISP™ Assay Kit
KF17316 25 Tests
EUR 291
FAM-Leu-CMK FLISP™ Assay Kit
KF17317 100 Tests
EUR 695
Caspase 12 Assay Kit
55R-1290 25 assays
EUR 328
Description: Assay Kit for detection of Capase 12 activity in the research laboratory
Caspase 3 Assay Kit
20-abx294003
  • EUR 1255.00
  • EUR 457.00
  • EUR 770.00
  • 100 tests
  • 20 tests
  • 50 tests
  • Shipped within 5-12 working days.
Poly-Caspase Assay Kit
20-abx299016
  • EUR 1302.00
  • EUR 582.00
  • 100 tests
  • 25 tests
  • Shipped within 1 week.
Caspase 1 Assay Kit
20-abx299019
  • EUR 1365.00
  • EUR 629.00
  • 100 tests
  • 25 tests
  • Shipped within 1 week.
Caspase 8 Assay Kit
20-abx299020
  • EUR 1302.00
  • EUR 582.00
  • 100 tests
  • 25 tests
  • Shipped within 1 week.
Caspase 9 Assay Kit
20-abx299021
  • EUR 1302.00
  • EUR 582.00
  • 100 tests
  • 25 tests
  • Shipped within 1 week.
Poly-Caspase Assay Kit
abx299022-2550tests 25-50 tests
EUR 692
  • Shipped within 1 week.
Caspase 1 Assay Kit
abx299023-2550tests 25-50 tests
EUR 692
  • Shipped within 1 week.
Poly-Caspase Assay Kit
20-abx299025
  • EUR 1302.00
  • EUR 582.00
  • 100 tests
  • 25 tests
  • Shipped within 1 week.
Caspase 2 Assay Kit
abx299026-25tests 25 tests
EUR 582
  • Shipped within 1 week.
Caspase 10 Assay Kit
abx299027-100tests 100 tests
EUR 1302
  • Shipped within 1 week.
Caspase 9 Assay Kit
20-abx299035
  • EUR 1302.00
  • EUR 582.00
  • 100 tests
  • 25 tests
  • Shipped within 1 week.
Aminooxy-5(6)-FAM
90103 5MG
EUR 242
Description: Minimum order quantity: 1 unit of 5MG
FAM azide, 6- isomer
A8118-25 25 mg
EUR 392
Description: FAM (fluorescein) azide. solid and 10 mM solution in DMSO, labeling reagent for Click chemistry.Pure 6-isomer.
FAM azide, 6- isomer
A8118-5 5 mg
EUR 166
Description: FAM (fluorescein) azide. solid and 10 mM solution in DMSO, labeling reagent for Click chemistry.Pure 6-isomer.
5(6)-FAM SE
HY-15937 100mg
EUR 215
Caspase 6 (Phospho-Ser257) Colorimetric Cell-Based ELISA Kit
EKC1975 100ul
EUR 572
6-carboxyfluorescein, se (6-fam, se)
90023 10MG
EUR 191
Description: Minimum order quantity: 1 unit of 10MG
Glycerol Cell-Based Assay Kit
K977-100
EUR 490
FAM-Spacer-Phe-CMK FLISP™ Assay Kit
KF17322 25 Tests
EUR 291
FAM-Spacer-Phe-CMK FLISP™ Assay Kit
KF17323 100 Tests
EUR 695
FAM-Spacer-Leu-CMK FLISP™ Assay Kit
KF17324 25 Tests
EUR 291
Caspase 3 Fluorometric Assay Kit
55R-1269 25 assays
EUR 277
Description: Assay Kit for detection of Capase 3 activity in the research laboratory
Caspase 3 Colorimetric Assay Kit
55R-1270 25 assays
EUR 277
Description: Assay Kit for detection of Capase 3 activity in the research laboratory
Caspase 1 Fluorometric Assay Kit
55R-1272 25 assays
EUR 277
Description: Assay Kit for detection of Capase 1 activity in the research laboratory
Caspase 1 Colorimetric Assay Kit
55R-1273 25 assays
EUR 277
Description: Assay Kit for detection of Capase 1 activity in the research laboratory
Caspase 8 Fluorometric Assay Kit
55R-1274 25 assays
EUR 277
Description: Assay Kit for detection of Capase 8 activity in the research laboratory
Caspase 8 Colorimetric Assay Kit
55R-1275 25 assays
EUR 277
Description: Assay Kit for detection of Capase 8 activity in the research laboratory
Caspase 2 Fluorometric Assay Kit
55R-1278 25 assays
EUR 284
Description: Assay Kit for detection of Capase 2 activity in the research laboratory
Caspase 2 Colorimetric Assay Kit
55R-1279 25 assays
EUR 303
Description: Assay Kit for detection of Capase 2 activity in the research laboratory
Caspase 9 Fluorometric Assay Kit
55R-1280 25 assays
EUR 277
Description: Assay Kit for detection of Capase 9 activity in the research laboratory
Caspase 9 Colorimetric Assay Kit
55R-1281 25 assays
EUR 296
Description: Assay Kit for detection of Capase 9 activity in the research laboratory
Caspase 5 Fluorometric Assay Kit
55R-1282 25 assays
EUR 284
Description: Assay Kit for detection of Capase 5 activity in the research laboratory
Caspase 5 Colorimetric Assay Kit
55R-1283 25 assays
EUR 303
Description: Assay Kit for detection of Capase 5 activity in the research laboratory
Caspase 10 Fluorometric Assay Kit
55R-1284 25 assays
EUR 284
Description: Assay Kit for detection of Capase 10 activity in the research laboratory
Caspase 10 Colorimetric Assay Kit
55R-1285 25 assays
EUR 303
Description: Assay Kit for detection of Capase 10 activity in the research laboratory
Caspase 4 Fluorometric Assay Kit
55R-1286 25 assays
EUR 303
Description: Assay Kit for detection of Capase 4 activity in the research laboratory
Caspase 4 Colorimetric Assay Kit
55R-1287 25 assays
EUR 312
Description: Assay Kit for detection of Capase 4 activity in the research laboratory
Caspase-1 Activity Assay Kit
AR4003-100Assays 100 Assays
EUR 514
Caspase-1 Activity Assay Kit
AR4003-25Assays 25 Assays
EUR 236
Caspase-1 Activity Assay Kit
AR4003-50Assays 50 Assays
EUR 340
Caspase-2 Activity Assay Kit
AR4004-100Assays 100 Assays
EUR 514
Caspase-2 Activity Assay Kit
AR4004-25Assays 25 Assays
EUR 236
Caspase-2 Activity Assay Kit
AR4004-50Assays 50 Assays
EUR 340
QuantiFluo Caspase-3 Assay Kit
DCS3-100 100
EUR 275
Description: Quantitative determination of the apoptosis target caspase-3 activity by fluorimetry (400/490nm) method. Procedure: 60 min. Kit size: 100 tests. Shelf life: 12 months.
Caspase 3/7 Assay Kit
20-abx299017
  • EUR 1302.00
  • EUR 582.00
  • 100 tests
  • 25 tests
  • Shipped within 1 week.
Caspase 3/7 Assay Kit
abx299024-2550tests 25-50 tests
EUR 692
  • Shipped within 1 week.
Caspase 3/7 Assay Kit
20-abx299028
  • EUR 1193.00
  • EUR 582.00
  • 100 tests
  • 25 tests
  • Shipped within 1 week.

A Preliminary Study on the Ability of the Trypsin-Like Peptidase Activity Assay Kit to Detect Periodontitis

 This study aimed to explore whether the Trypsin-Like Peptidase Activity Assay Kit (TLP-AA-Kit), which measures the activity of N-benzoyl-dl-arginine peptidase (trypsin-like peptidase), can be used as a reliable tool for periodontitis detection in population-based surveillance. In total, 105 individuals underwent a full-mouth periodontal examination and provided tongue swabs as specimens for further analyses. The results of the TLP-AA-Kit were scored between 1 and 5; higher scores indicated higher trypsin concentrations. Receiver operating characteristic analyses were used to evaluate the predictive validity of the TLP-AA-Kit, where the periodontitis case definition provided by the Centers for Disease Control/American Academy of Periodontology served as the reference.

Severe and moderate periodontitis were identified in 4.8% and 16.2% of the study population, respectively. The TLP-AA-Kit showed high diagnostic accuracy for severe periodontitis, with an area under the curve of 0.93 (95% confidence interval = 0.88-0.99). However, the diagnostic accuracy of the TLP-AA-Kit for moderate/severe periodontitis was not reliable. While further studies are necessary to validate our results, the results provided herein highlight the potential of the TLP-AA-Kit as a useful tool for the detection of periodontitis, particularly in severe cases, for population-based surveillance.

Comparative Performance of SARS-CoV-2 Detection Assays using Seven Different Primer/Probe Sets and One Assay Kit 

More than 100,000 people worldwide are known to have been infected with SARS-CoV-2 beginning in December 2019. The virus has now spread to over 93 countries including the United States, with the largest cluster of US cases to date in the Seattle metropolitan area in Washington. Given the rapid increase in the number of local cases, the availability of accurate, high-throughput SARS-CoV-2 testing is vital to efforts to manage the current public health crisis. In the course of optimizing SARS-CoV-2 testing performed by the University of Washington Clinical Virology Lab (UW Virology Lab), we tested assays using seven different primer/probe sets and one assay kit.

We found that the most sensitive assays were those the used the E-gene primer/probe set described by Corman et al. (Eurosurveillance 25(3), 2020, https://doi.org/10.2807/1560-7917.ES.2020.25.3.2000045) and the N2 set described by the CDC (Division of Viral Diseases, Centers for Disease Control and Prevention, 2020, https://www.cdc.gov/coronavirus/2019-ncov/downloads/rt-pcr-panel-primer-probes.pdf). All assays tested were found to be highly specific for SARS-CoV-2, with no cross-reactivity with other respiratory viruses observed in our analyses regardless of the primer/probe set or kit used. These results will provide invaluable information to other clinical laboratories who are actively developing SARS-CoV-2 testing protocols at a time when increased testing capacity is urgently needed worldwide.

Comparative Performance of SARS-CoV-2 Detection Assays using Seven Different Primer/Probe Sets and One Assay Kit.

 Nearly 400,000 people worldwide are known to have been infected with SARS-CoV-2 beginning in December 2019. The virus has now spread to over 168 countries including the United States, where the first cluster of cases was observed in the Seattle metropolitan area in Washington. Given the rapid increase in the number of cases in many localities, the availability of accurate, high-throughput SARS-CoV-2 testing is vital to efforts to manage the current public health crisis.

In the course of optimizing SARS-CoV-2 testing performed by the University of Washington Clinical Virology Lab (UW Virology Lab), we evaluated assays using seven different primer/probe sets and one assay kit. We found that the most sensitive assays were those that used the E-gene primer/probe set described by Corman et al. (Eurosurveillance 25 (3), 2020, https://doi.org/10.2807/1560-7917.ES.2020.25.3.2000045) and the N2 set developed by the CDC (Division of Viral Diseases, Centers for Disease Control and Prevention, 2020,

All assays tested were found to be highly specific for SARS-CoV-2, with no cross-reactivity with other respiratory viruses observed in our analyses regardless of the primer/probe set or kit used. These results will provide valuable information to other clinical laboratories who are actively developing SARS-CoV-2 testing protocols at a time when increased testing capacity is urgently needed worldwide.

Development and validation of a rapid kit for authenticity of murine meat in meat products with a species-specific PCR assay.

 Adulteration of meat products with murine meat poses a huge threat to consumer health and leads to serious disruption in food markets. Species authentication of murine meat is still technically challenging. We, therefore, developed a species-specific PCR kit consisting of murine meat DNA extraction, PCR reaction and identifying systems. We designed novel universal primers targeting highly conserved region on mitochondrial cytochrome b gene (cyt b) from four murines (lab rats, lab mice, wild rat and wild mice), as well as specific primers for meat from four widely consumed animal species, cattle, sheep, duck and donkey. Simultaneously, pasmid inserted specific cyt b fragment was cloned and used as the internal positve control in the kit. The kit parameters of specificity, sensitivity, stability and validity were determined using mimic counterfeiting meatball.

The specificity of the DNA detection kit was 100% in authentication of the four fraudulent meats of cattle, sheep, duck and donkey mixed murine meat. The minimum detection limit of the sample DNA was 0.1 μg. The kit, which had freeze-thawed up to 20 times and stored for 1 year, also was powerful in detecting an amount of 0.1 mg in artificial counterfeited cattle, sheep, duck and donkey meat products. The murine-species DNA detection kit proposed in this study has proved to be a simple, accurate and effective assay, and can be applied to the identification of murine meat traces in common edible meat, to ensure the realisable implementation of meat product market supervision.

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