Comparison of an Immunochromatographic Assay Kit

oxfordgenomesciences

Comparison of an Immunochromatographic Assay Kit with DAS-ELISA for Large-Scale Diagnosis and Molecular Discrimination of Satsuma Dwarf Virus Collected from Citrus Orchards 

Satsuma dwarf virus (SDV) seriously damages citrus production by reducing the quality and yield of fruit. To avoid contamination with SDV, mother trees are checked to be SDV-free in advance of nursery tree distribution. In this study, we compared an immunochromatographic assay (ICA) kit with double-antibody sandwich enzyme-linked immunosorbent assay (DASELISA) for large-scale diagnosis of SDV in orchardgrown trees in Shizuoka Prefecture, Japan. The two methods gave conflicting results for 11 of 1,705 samples, all of which were negative by DAS-ELISA but positive by ICA.

The samples scored as positive by either DASELISA or ICA were analyzed by reverse transcription polymerase chain reaction and all were confirmed to be positive. These results validate the use of ICA as a screening method for large-scale diagnosis. Strain discrimination revealed that 16 of 22 isolates belonged to SDV, while citrus mosaic virus (CiMV) infection only and co-infection (SDV and CiMV) were in a minority.

oxfordgenomesciences
oxfordgenomesciences

Caspase 6 Fluorometric Assay Kit

55R-1276 25 assays
EUR 344.4
Description: Assay Kit for detection of Capase 6 activity in the research laboratory

Caspase 6 Colorimetric Assay Kit

55R-1277 25 assays
EUR 340.8
Description: Assay Kit for detection of Capase 6 activity in the research laboratory

Caspase 6 Assay Kit, green

KF17204 25 Tests
EUR 364.8

Caspase-6 Fluorometric Assay Kit

K114-100
EUR 516

Caspase-6 Fluorometric Assay Kit

K114-200
EUR 738

Caspase-6 Fluorometric Assay Kit

K114-25
EUR 242.4

Caspase-6 Fluorometric Assay Kit

K114-400
EUR 1149.6

Caspase-6 Colorimetric Assay Kit

K115-100
EUR 542.4

Caspase-6 Colorimetric Assay Kit

K115-200
EUR 738

Caspase-6 Colorimetric Assay Kit

K115-25
EUR 242.4

Caspase-6 Colorimetric Assay Kit

K115-400
EUR 1149.6

Caspase-6 Fluorometric Assay Kit

K2014-100 100 assays
EUR 568.8

Caspase-6 Fluorometric Assay Kit

K2014-200 200 assays
EUR 835.2

Caspase-6 Fluorometric Assay Kit

K2014-25 25 assays
EUR 285.6

Caspase-6 Fluorometric Assay Kit

K2014-400 400 assays
EUR 1303.2

Caspase-6 Colorimetric Assay Kit

K2015-100 100 assays
EUR 584.4

Caspase-6 Colorimetric Assay Kit

K2015-200 200 assays
EUR 835.2

Caspase-6 Colorimetric Assay Kit

K2015-25 25 assays
EUR 285.6

Caspase-6 Colorimetric Assay Kit

K2015-400 400 assays
EUR 1321.2

6-FAM

HY-66021 500mg
EUR 374.4

Caspase 6 Colorimetric Cell-Based ELISA Kit

EKC1087 100ul
EUR 686.4

Genorise® Caspase-6 Colorimetric Assay Kit

GR107023 100 tests
EUR 346.8

Genorise® Caspase-6 Fluorometric Assay Kit

GR107024 100 tests
EUR 346.8

6 FAM SE

A8771-10 10 mg
EUR 208.8
Description: 6-FAM SE, also known as 6-Carboxyfluorescein N-hydroxysuccinimide ester or 6-Carboxyfluorescein N-succinimidyl ester, is an isomer of carboxyfluorescein and amine-reactive succinimidyl ester carboxyfluorescein.

6 FAM SE

A8771-100 100 mg
EUR 877.2
Description: 6-FAM SE, also known as 6-Carboxyfluorescein N-hydroxysuccinimide ester or 6-Carboxyfluorescein N-succinimidyl ester, is an isomer of carboxyfluorescein and amine-reactive succinimidyl ester carboxyfluorescein.

6 FAM SE

A8771-5.1 10 mM (in 1mL DMSO)
EUR 238.8
Description: 6-FAM SE, also known as 6-Carboxyfluorescein N-hydroxysuccinimide ester or 6-Carboxyfluorescein N-succinimidyl ester, is an isomer of carboxyfluorescein and amine-reactive succinimidyl ester carboxyfluorescein.

6 FAM SE

A8771-50 50 mg
EUR 543.6
Description: 6-FAM SE, also known as 6-Carboxyfluorescein N-hydroxysuccinimide ester or 6-Carboxyfluorescein N-succinimidyl ester, is an isomer of carboxyfluorescein and amine-reactive succinimidyl ester carboxyfluorescein.

6-FAM Azide

955 100 mg
EUR 842.4

6-FAM Alkyne

956 100 mg
EUR 842.4

6-FAM SE

HY-15939 100mg
EUR 734.4

FAM FLICAâ„¢ POLY CASPASE KIT (OKSA11268)

OKSA11268 25 Tests
EUR 709.2
Description: Description of target: FAM FLICAâ„¢ POLY CASPASE KIT;Species reactivity: ;Application: FC, IF;Assay info: ;Sensitivity:

FAM FLICAâ„¢ POLY CASPASE KIT (OKSA11269)

OKSA11269 100 Tests
EUR 1828.8
Description: Description of target: FAM FLICAâ„¢ POLY CASPASE KIT;Species reactivity: ;Application: FC, IF;Assay info: ;Sensitivity:

FAM FLICAâ„¢ CASPASE-8 KIT (OKSA11276)

OKSA11276 25 Tests
EUR 709.2
Description: Description of target: FAM FLICAâ„¢ CASPASE-8 KIT;Species reactivity: ;Application: FC, IF;Assay info: ;Sensitivity:

FAM FLICAâ„¢ CASPASE-8 KIT (OKSA11277)

OKSA11277 100 Tests
EUR 1828.8
Description: Description of target: FAM FLICAâ„¢ CASPASE-8 KIT;Species reactivity: ;Application: FC, IF;Assay info: ;Sensitivity:

FAM FLICAâ„¢ CASPASE-9 KIT (OKSA11278)

OKSA11278 25 Tests
EUR 709.2
Description: Description of target: FAM FLICAâ„¢ CASPASE-9 KIT;Species reactivity: ;Application: FC, IF;Assay info: ;Sensitivity:

FAM FLICAâ„¢ CASPASE-9 KIT (OKSA11279)

OKSA11279 100 Tests
EUR 1828.8
Description: Description of target: FAM FLICAâ„¢ CASPASE-9 KIT;Species reactivity: ;Application: FC, IF;Assay info: ;Sensitivity:

FAM FLICAâ„¢ CASPASE-2 KIT (OKSA11282)

OKSA11282 25 Tests
EUR 668.4
Description: Description of target: FAM FLICAâ„¢ CASPASE-2 KIT;Species reactivity: ;Application: FC, IF;Assay info: ;Sensitivity:

Caspase 6 Colorimetric Cell-Based ELISA Kit (OKAG00581)

OKAG00581 96 Wells
EUR 715.2
Description: Description of target: ;Species reactivity: Human, Mouse, Rat;Application: ELISA;Assay info: Assay Type: Cell-Based
Subtype: None
Detection Method: Colorimetric 450 nm;Sensitivity:

FAM-Phe-CMK Serine Protease Assay Kit

abx299010-25tests 25 tests
EUR 679.2

FAM-Leu-CMK Serine Protease Assay Kit

abx299011-25tests 25 tests
EUR 679.2

FAM-Leu-DAP Serine Protease Assay Kit

abx299012-25tests 25 tests
EUR 679.2

FAM-Phe-DAP Serine Protease Assay Kit

abx299013-25tests 25 tests
EUR 698.4

FAM-Phe-CMK FLISP™ Assay Kit

KF17314 25 Tests
EUR 349.2

FAM-Phe-CMK FLISP™ Assay Kit

KF17315 100 Tests
EUR 834

FAM-Lue-CMK FLISP™ Assay Kit

KF17316 25 Tests
EUR 349.2

FAM-Leu-CMK FLISP™ Assay Kit

KF17317 100 Tests
EUR 834

Caspase 12 Assay Kit

55R-1290 25 assays
EUR 393.6
Description: Assay Kit for detection of Capase 12 activity in the research laboratory

Caspase 3 Assay Kit

20-abx294003
  • EUR 1506.00
  • EUR 548.40
  • EUR 924.00
  • 100 tests
  • 20 tests
  • 50 tests

Poly-Caspase Assay Kit

20-abx299016
  • EUR 1562.40
  • EUR 698.40
  • 100 tests
  • 25 tests

Caspase 1 Assay Kit

20-abx299019
  • EUR 1638.00
  • EUR 754.80
  • 100 tests
  • 25 tests

Caspase 8 Assay Kit

20-abx299020
  • EUR 1562.40
  • EUR 698.40
  • 100 tests
  • 25 tests

Caspase 9 Assay Kit

20-abx299021
  • EUR 1562.40
  • EUR 698.40
  • 100 tests
  • 25 tests

Poly-Caspase Assay Kit

abx299022-2550tests 25-50 tests
EUR 830.4

Caspase 1 Assay Kit

abx299023-2550tests 25-50 tests
EUR 830.4

Poly-Caspase Assay Kit

20-abx299025
  • EUR 1562.40
  • EUR 698.40
  • 100 tests
  • 25 tests

Caspase 2 Assay Kit

abx299026-25tests 25 tests
EUR 698.4

Caspase 10 Assay Kit

abx299027-100tests 100 tests
EUR 1562.4

Caspase 9 Assay Kit

20-abx299035
  • EUR 1562.40
  • EUR 698.40
  • 100 tests
  • 25 tests

Aminooxy-5(6)-FAM

90103 5MG
EUR 290.4
Description: Minimum order quantity: 1 unit of 5MG

FAM azide, 6- isomer

A8118-25 25 mg
EUR 470.4
Description: FAM (fluorescein) azide. solid and 10 mM solution in DMSO, labeling reagent for Click chemistry.Pure 6-isomer.

FAM azide, 6- isomer

A8118-5 5 mg
EUR 199.2
Description: FAM (fluorescein) azide. solid and 10 mM solution in DMSO, labeling reagent for Click chemistry.Pure 6-isomer.

5(6)-FAM SE

HY-15937 100mg
EUR 258

Caspase 6 (Phospho-Ser257) Colorimetric Cell-Based ELISA Kit

EKC1975 100ul
EUR 686.4

6-carboxyfluorescein, se (6-fam, se)

90023 10MG
EUR 229.2
Description: Minimum order quantity: 1 unit of 10MG

Glycerol Cell-Based Assay Kit

K977-100
EUR 588

FAM-Spacer-Phe-CMK FLISP™ Assay Kit

KF17322 25 Tests
EUR 349.2

FAM-Spacer-Phe-CMK FLISP™ Assay Kit

KF17323 100 Tests
EUR 834

FAM-Spacer-Leu-CMK FLISP™ Assay Kit

KF17324 25 Tests
EUR 349.2

Caspase 3 Fluorometric Assay Kit

55R-1269 25 assays
EUR 332.4
Description: Assay Kit for detection of Capase 3 activity in the research laboratory

Caspase 3 Colorimetric Assay Kit

55R-1270 25 assays
EUR 332.4
Description: Assay Kit for detection of Capase 3 activity in the research laboratory

Caspase 1 Fluorometric Assay Kit

55R-1272 25 assays
EUR 332.4
Description: Assay Kit for detection of Capase 1 activity in the research laboratory

Caspase 1 Colorimetric Assay Kit

55R-1273 25 assays
EUR 332.4
Description: Assay Kit for detection of Capase 1 activity in the research laboratory

Caspase 8 Fluorometric Assay Kit

55R-1274 25 assays
EUR 332.4
Description: Assay Kit for detection of Capase 8 activity in the research laboratory

Caspase 8 Colorimetric Assay Kit

55R-1275 25 assays
EUR 332.4
Description: Assay Kit for detection of Capase 8 activity in the research laboratory

Caspase 2 Fluorometric Assay Kit

55R-1278 25 assays
EUR 340.8
Description: Assay Kit for detection of Capase 2 activity in the research laboratory

Caspase 2 Colorimetric Assay Kit

55R-1279 25 assays
EUR 363.6
Description: Assay Kit for detection of Capase 2 activity in the research laboratory

Caspase 9 Fluorometric Assay Kit

55R-1280 25 assays
EUR 332.4
Description: Assay Kit for detection of Capase 9 activity in the research laboratory

Caspase 9 Colorimetric Assay Kit

55R-1281 25 assays
EUR 355.2
Description: Assay Kit for detection of Capase 9 activity in the research laboratory

Caspase 5 Fluorometric Assay Kit

55R-1282 25 assays
EUR 340.8
Description: Assay Kit for detection of Capase 5 activity in the research laboratory

Caspase 5 Colorimetric Assay Kit

55R-1283 25 assays
EUR 363.6
Description: Assay Kit for detection of Capase 5 activity in the research laboratory

Caspase 10 Fluorometric Assay Kit

55R-1284 25 assays
EUR 340.8
Description: Assay Kit for detection of Capase 10 activity in the research laboratory

Caspase 10 Colorimetric Assay Kit

55R-1285 25 assays
EUR 363.6
Description: Assay Kit for detection of Capase 10 activity in the research laboratory

Caspase 4 Fluorometric Assay Kit

55R-1286 25 assays
EUR 363.6
Description: Assay Kit for detection of Capase 4 activity in the research laboratory

Caspase 4 Colorimetric Assay Kit

55R-1287 25 assays
EUR 374.4
Description: Assay Kit for detection of Capase 4 activity in the research laboratory

Caspase 3/7 Assay Kit

20-abx299017
  • EUR 1562.40
  • EUR 698.40
  • 100 tests
  • 25 tests

Caspase 3/7 Assay Kit

abx299024-2550tests 25-50 tests
EUR 830.4

Caspase 3/7 Assay Kit

20-abx299028
  • EUR 1431.60
  • EUR 698.40
  • 100 tests
  • 25 tests

Caspase-1 Activity Assay Kit

AR4003-100Assays 100 Assays
EUR 616.8

Caspase-1 Activity Assay Kit

AR4003-25Assays 25 Assays
EUR 283.2

Caspase-1 Activity Assay Kit

AR4003-50Assays 50 Assays
EUR 408

Caspase-2 Activity Assay Kit

AR4004-100Assays 100 Assays
EUR 616.8

Caspase-2 Activity Assay Kit

AR4004-25Assays 25 Assays
EUR 283.2

Caspase-2 Activity Assay Kit

AR4004-50Assays 50 Assays
EUR 408

QuantiFluo Caspase-3 Assay Kit

DCS3-100 100
EUR 330
Description: Quantitative determination of the apoptosis target caspase-3 activity by fluorimetry (400/490nm) method. Procedure: 60 min. Kit size: 100 tests. Shelf life: 12 months.

Caspase-5 Fluorometric Assay Kit

K2195-100 100 assays
EUR 602.4

Caspase-5 Fluorometric Assay Kit

K2195-200 200 assays
EUR 835.2

Caspase-5 Fluorometric Assay Kit

K2195-25 25 assays
EUR 285.6

Caspase-5 Fluorometric Assay Kit

K2195-400 400 assays
EUR 1336.8

Caspase-5 Colorimetric Assay Kit

K2196-100 100 assays
EUR 634.8

Caspase-5 Colorimetric Assay Kit

K2196-200 200 assays
EUR 870

Caspase-5 Colorimetric Assay Kit

K2196-25 25 assays
EUR 301.2

Caspase-5 Colorimetric Assay Kit

K2196-400 400 assays
EUR 1387.2

Caspase-10 Colorimetric Assay Kit

K2197-100 100 assays
EUR 634.8

Caspase-10 Colorimetric Assay Kit

K2197-200 200 assays
EUR 885.6

Caspase-10 Colorimetric Assay Kit

K2197-25 25 assays
EUR 301.2

Caspase-10 Colorimetric Assay Kit

K2197-400 400 assays
EUR 1336.8

Caspase-4 Fluorometric Assay Kit

K2198-100 100 assays
EUR 634.8

Caspase-4 Fluorometric Assay Kit

K2198-200 200 assays
EUR 885.6

Caspase-4 Fluorometric Assay Kit

K2198-25 25 assays
EUR 301.2

Caspase-4 Fluorometric Assay Kit

K2198-400 400 assays
EUR 1336.8

Caspase-4 Colorimetric Assay Kit

K2199-100 100 assays
EUR 668.4

Caspase-4 Colorimetric Assay Kit

K2199-200 200 assays
EUR 885.6

Caspase-4 Colorimetric Assay Kit

K2199-25 25 assays
EUR 319.2

Caspase-4 Colorimetric Assay Kit

K2199-400 400 assays
EUR 1336.8

Caspase 1 Assay Kit, green

KF17201 25 Tests
EUR 390

Caspase 2 Assay Kit, green

KF17202 25 Tests
EUR 364.8

Caspase 8 Assay Kit, green

KF17205 25 Tests
EUR 364.8

Caspase 9 Assay Kit, green

KF17206 25 Tests
EUR 364.8

Caspase 10 Assay Kit, green

KF17207 25 Tests
EUR 364.8

Caspase 13 Assay Kit, green

KF17208 25 Tests
EUR 364.8

Caspase 9 Assay Kit, red

KF17302 25 Tests
EUR 364.8

660 Caspase-1 Assay Kit

KF17362 25-50 Tests
EUR 418.8

Caspase-12 Fluorometric Assay Kit

K139-100
EUR 601.2

Caspase-12 Fluorometric Assay Kit

K139-25
EUR 307.2

Caspase-4 Fluorometric Assay Kit

K126-100
EUR 561.6

Caspase-4 Fluorometric Assay Kit

K126-200
EUR 738

Caspase-4 Fluorometric Assay Kit

K126-25
EUR 255.6

Caspase-4 Fluorometric Assay Kit

K126-400
EUR 1110

Caspase-4 Colorimetric Assay Kit

K127-100
EUR 561.6

Caspase-4 Colorimetric Assay Kit

K127-200
EUR 738

Caspase-4 Colorimetric Assay Kit

K127-25
EUR 274.8

Caspase-4 Colorimetric Assay Kit

K127-400
EUR 1149.6

Caspase-5 Fluorometric Assay Kit

K122-100
EUR 523.2

Caspase-5 Fluorometric Assay Kit

K122-200
EUR 698.4

Caspase-5 Fluorometric Assay Kit

K122-25
EUR 242.4

Caspase-5 Fluorometric Assay Kit

K122-400
EUR 1149.6

Caspase-5 Colorimetric Assay Kit

K123-100
EUR 561.6

Caspase-5 Colorimetric Assay Kit

K123-200
EUR 738

Caspase-5 Colorimetric Assay Kit

K123-25
EUR 255.6

Caspase-5 Colorimetric Assay Kit

K123-400
EUR 1149.6

Caspase-10 Fluorometric Assay Kit

K124-100
EUR 529.2

Caspase-10 Fluorometric Assay Kit

K124-200
EUR 738

Caspase-10 Fluorometric Assay Kit

K124-25
EUR 242.4

Caspase-10 Fluorometric Assay Kit

K124-400
EUR 1149.6

Caspase-10 Colorimetric Assay Kit

K125-100
EUR 555.6

Caspase-10 Colorimetric Assay Kit

K125-200
EUR 738

Caspase-10 Colorimetric Assay Kit

K125-25
EUR 255.6

Caspase-10 Colorimetric Assay Kit

K125-400
EUR 1149.6

Caspase-8 Fluorometric Assay Kit

K112-100
EUR 574.8

Caspase-8 Fluorometric Assay Kit

K112-200
EUR 738

Caspase-8 Fluorometric Assay Kit

K112-25
EUR 248.4

Caspase-8 Fluorometric Assay Kit

K112-400
EUR 1149.6

Caspase-8 Colorimetric Assay Kit

K113-100
EUR 574.8

Caspase-8 Colorimetric Assay Kit

K113-200
EUR 744

Caspase-8 Colorimetric Assay Kit

K113-25
EUR 248.4

Caspase-8 Colorimetric Assay Kit

K113-400
EUR 1149.6

Caspase-2 Fluorometric Assay Kit

K116-100
EUR 555.6

Caspase-2 Fluorometric Assay Kit

K116-200
EUR 738

Caspase-2 Fluorometric Assay Kit

K116-25
EUR 242.4

Caspase-2 Fluorometric Assay Kit

K116-400
EUR 1149.6

A Preliminary Study on the Ability of the Trypsin-Like Peptidase Activity Assay Kit to Detect Periodontitis

 This study aimed to explore whether the Trypsin-Like Peptidase Activity Assay Kit (TLP-AA-Kit), which measures the activity of N-benzoyl-dl-arginine peptidase (trypsin-like peptidase), can be used as a reliable tool for periodontitis detection in population-based surveillance. In total, 105 individuals underwent a full-mouth periodontal examination and provided tongue swabs as specimens for further analyses. The results of the TLP-AA-Kit were scored between 1 and 5; higher scores indicated higher trypsin concentrations. Receiver operating characteristic analyses were used to evaluate the predictive validity of the TLP-AA-Kit, where the periodontitis case definition provided by the Centers for Disease Control/American Academy of Periodontology served as the reference.

Severe and moderate periodontitis were identified in 4.8% and 16.2% of the study population, respectively. The TLP-AA-Kit showed high diagnostic accuracy for severe periodontitis, with an area under the curve of 0.93 (95% confidence interval = 0.88-0.99). However, the diagnostic accuracy of the TLP-AA-Kit for moderate/severe periodontitis was not reliable. While further studies are necessary to validate our results, the results provided herein highlight the potential of the TLP-AA-Kit as a useful tool for the detection of periodontitis, particularly in severe cases, for population-based surveillance.

Comparative Performance of SARS-CoV-2 Detection Assays using Seven Different Primer/Probe Sets and One Assay Kit 

More than 100,000 people worldwide are known to have been infected with SARS-CoV-2 beginning in December 2019. The virus has now spread to over 93 countries including the United States, with the largest cluster of US cases to date in the Seattle metropolitan area in Washington. Given the rapid increase in the number of local cases, the availability of accurate, high-throughput SARS-CoV-2 testing is vital to efforts to manage the current public health crisis. In the course of optimizing SARS-CoV-2 testing performed by the University of Washington Clinical Virology Lab (UW Virology Lab), we tested assays using seven different primer/probe sets and one assay kit.

We found that the most sensitive assays were those the used the E-gene primer/probe set described by Corman et al. (Eurosurveillance 25(3), 2020, https://doi.org/10.2807/1560-7917.ES.2020.25.3.2000045) and the N2 set described by the CDC (Division of Viral Diseases, Centers for Disease Control and Prevention, 2020, https://www.cdc.gov/coronavirus/2019-ncov/downloads/rt-pcr-panel-primer-probes.pdf). All assays tested were found to be highly specific for SARS-CoV-2, with no cross-reactivity with other respiratory viruses observed in our analyses regardless of the primer/probe set or kit used. These results will provide invaluable information to other clinical laboratories who are actively developing SARS-CoV-2 testing protocols at a time when increased testing capacity is urgently needed worldwide.

Comparative Performance of SARS-CoV-2 Detection Assays using Seven Different Primer/Probe Sets and One Assay Kit.

 Nearly 400,000 people worldwide are known to have been infected with SARS-CoV-2 beginning in December 2019. The virus has now spread to over 168 countries including the United States, where the first cluster of cases was observed in the Seattle metropolitan area in Washington. Given the rapid increase in the number of cases in many localities, the availability of accurate, high-throughput SARS-CoV-2 testing is vital to efforts to manage the current public health crisis.

In the course of optimizing SARS-CoV-2 testing performed by the University of Washington Clinical Virology Lab (UW Virology Lab), we evaluated assays using seven different primer/probe sets and one assay kit. We found that the most sensitive assays were those that used the E-gene primer/probe set described by Corman et al. (Eurosurveillance 25 (3), 2020, https://doi.org/10.2807/1560-7917.ES.2020.25.3.2000045) and the N2 set developed by the CDC (Division of Viral Diseases, Centers for Disease Control and Prevention, 2020,

All assays tested were found to be highly specific for SARS-CoV-2, with no cross-reactivity with other respiratory viruses observed in our analyses regardless of the primer/probe set or kit used. These results will provide valuable information to other clinical laboratories who are actively developing SARS-CoV-2 testing protocols at a time when increased testing capacity is urgently needed worldwide.

Development and validation of a rapid kit for authenticity of murine meat in meat products with a species-specific PCR assay.

 Adulteration of meat products with murine meat poses a huge threat to consumer health and leads to serious disruption in food markets. Species authentication of murine meat is still technically challenging. We, therefore, developed a species-specific PCR kit consisting of murine meat DNA extraction, PCR reaction and identifying systems. We designed novel universal primers targeting highly conserved region on mitochondrial cytochrome b gene (cyt b) from four murines (lab rats, lab mice, wild rat and wild mice), as well as specific primers for meat from four widely consumed animal species, cattle, sheep, duck and donkey. Simultaneously, pasmid inserted specific cyt b fragment was cloned and used as the internal positve control in the kit. The kit parameters of specificity, sensitivity, stability and validity were determined using mimic counterfeiting meatball.

The specificity of the DNA detection kit was 100% in authentication of the four fraudulent meats of cattle, sheep, duck and donkey mixed murine meat. The minimum detection limit of the sample DNA was 0.1 μg. The kit, which had freeze-thawed up to 20 times and stored for 1 year, also was powerful in detecting an amount of 0.1 mg in artificial counterfeited cattle, sheep, duck and donkey meat products. The murine-species DNA detection kit proposed in this study has proved to be a simple, accurate and effective assay, and can be applied to the identification of murine meat traces in common edible meat, to ensure the realisable implementation of meat product market supervision.

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