Comparison of an Immunochromatographic Assay Kit

oxfordgenomesciences

Comparison of an Immunochromatographic Assay Kit with DAS-ELISA for Large-Scale Diagnosis and Molecular Discrimination of Satsuma Dwarf Virus Collected from Citrus Orchards 

Satsuma dwarf virus (SDV) seriously damages citrus production by reducing the quality and yield of fruit. To avoid contamination with SDV, mother trees are checked to be SDV-free in advance of nursery tree distribution. In this study, we compared an immunochromatographic assay (ICA) kit with double-antibody sandwich enzyme-linked immunosorbent assay (DASELISA) for large-scale diagnosis of SDV in orchardgrown trees in Shizuoka Prefecture, Japan. The two methods gave conflicting results for 11 of 1,705 samples, all of which were negative by DAS-ELISA but positive by ICA.

The samples scored as positive by either DASELISA or ICA were analyzed by reverse transcription polymerase chain reaction and all were confirmed to be positive. These results validate the use of ICA as a screening method for large-scale diagnosis. Strain discrimination revealed that 16 of 22 isolates belonged to SDV, while citrus mosaic virus (CiMV) infection only and co-infection (SDV and CiMV) were in a minority.

oxfordgenomesciences
oxfordgenomesciences

FAM FLICA CASPASE-1 KIT

GWB-Q00437 25 TESTS Ask for price

FAM FLICA CASPASE-1 KIT

GWB-Q00438 100 TESTS Ask for price

FAM FLICA CASPASE-2 KIT

GWB-Q00441 25 TESTS Ask for price

FAM FLICA CASPASE-2 KIT

GWB-Q00442 100 TESTS Ask for price

FAM FLICA CASPASE-8 KIT

GWB-Q00447 25 TESTS Ask for price

FAM FLICA CASPASE-8 KIT

GWB-Q00448 100 TESTS Ask for price

FAM FLICA CASPASE-9 KIT

GWB-Q00449 25 TESTS Ask for price

FAM FLICA CASPASE-9 KIT

GWB-Q00450 100 TESTS Ask for price

FAM FLICA POLY CASPASE KIT

GWB-Q00451 25 TESTS Ask for price

FAM FLICA POLY CASPASE KIT

GWB-Q00452 100 TESTS Ask for price

FAM FLICA CASPASE-3/7 KIT

GWB-Q00443 25 TESTS Ask for price

FAM FLICA CASPASE-3/7 KIT

GWB-Q00444 100 TESTS Ask for price

OKSA11272-25T - FAM FLICA™ CASPASE-6 KIT

OKSA11272-25T 25Tests
EUR 469

SR FLICA® Caspase 3 and 7 Assay Kit

931 25
EUR 216

SR FLICA® Caspase 3 and 7 Assay Kit

932 100
EUR 615

OKSA11273-100T - FAM FLICA™ CASPASE-6 KIT

OKSA11273-100T 100Tests
EUR 1269

FAM FLICA CASPASE-10 KIT (OKSA11285)

OKSA11285 100 Tests
EUR 1875.6
Description: Description of target: FAM FLICAâ„¢ CASPASE-10 KIT;Species reactivity: ;Application: FC, IF;Assay info: ;Sensitivity:

FAM FLICA CASPASE-1 KIT (OKSA11274)

OKSA11274 25 Tests
EUR 709.2
Description: Description of target: FAM FLICAâ„¢ CASPASE-1 KIT;Species reactivity: ;Application: FC, IF;Assay info: ;Sensitivity:

FAM FLICA CASPASE-1 KIT (OKSA11275)

OKSA11275 100 Tests
EUR 1827.6
Description: Description of target: The FAM FLICATM Capase 1 kit uses a target sequence (YVAD) sandwiched between a green fluorescent label, FAM, and FMK to make a quick and flexible method to analyze active capases in apoptotic cells. These kits measure apoptosis by detecting active caspases in whole, living cells. They don't work by using antibodies or an ELISA. Instead, their methodology is based a unique cell-permeable and non-cytotoxic reagent called Fluorochrome Inhibitor of Caspases. These kits are suitable for cells in suspension, adherent cells, thin tissue sections (but not fixed or paraffin-embedded cells) from many species including mammalian, insect, and yeast. Different cell types, e.g. HeLa, primary neurons, macrophages and lymphocytes have also been successfully studied with these kits. ;Species reactivity: Human;Application: FC, IF;Assay info: ;Sensitivity:

FAM FLICA CASPASE-2 KIT (OKSA11283)

OKSA11283 100 Tests
EUR 1593.6
Description: Description of target: FAM FLICAâ„¢ CASPASE-2 KIT;Species reactivity: ;Application: FC, IF;Assay info: ;Sensitivity:

FAM FLICA CASPASE-3/7 KIT (OKSA11271)

OKSA11271 100 Tests
EUR 1828.8
Description: Description of target: The FAM FLICA™ Caspase 3 & 7 kit uses a quick and easy method to analyze active caspases in apoptotic cells.;Species reactivity: ;Application: FC, IF;Assay info: ;Sensitivity:

OKSA11274-25T - FAM FLICA CASPASE-1 KIT

OKSA11274-25T 25Tests
EUR 459

FAM-FLICA® Caspase 9 Assay Kit

912 25
EUR 216

FAM-FLICA® Caspase 9 Assay Kit

913 100
EUR 615

FAM-FLICA® Caspase 2 Assay Kit

918 25
EUR 216

FAM-FLICA® Caspase 2 Assay Kit

919 100
EUR 615

FAM-FLICA® Caspase 10 Assay Kit

922 25
EUR 216

OKSA11285-100T - FAM FLICA CASPASE-10 KIT

OKSA11285-100T 100Tests
EUR 1279

OKSA11275-100T - FAM FLICA CASPASE-1 KIT

OKSA11275-100T 100Tests
EUR 1249

OKSA11276-25T - FAM FLICA™ CASPASE-8 KIT

OKSA11276-25T 25Tests
EUR 459

OKSA11278-25T - FAM FLICA™ CASPASE-9 KIT

OKSA11278-25T 25Tests
EUR 459

OKSA11271-100T - FAM FLICA CASPASE-3/7 KIT

OKSA11271-100T 100Tests
EUR 1249

OKSA11277-100T - FAM FLICA™ CASPASE-8 KIT

OKSA11277-100T 100Tests
EUR 1249

OKSA11279-100T - FAM FLICA™ CASPASE-9 KIT

OKSA11279-100T 100Tests
EUR 1249

OKSA11268-25T - FAM FLICA™ POLY CASPASE KIT

OKSA11268-25T 25Tests
EUR 459

OKSA11269-100T - FAM FLICA™ POLY CASPASE KIT

OKSA11269-100T 100Tests
EUR 1249

FAM-FLICA® Caspase 1 WEHD Assay Kit

9161 25
EUR 234

FAM-FLICA® Caspase 1 WEHD Assay Kit

9162 100
EUR 649

FAM FLICAâ„¢ CASPASE-6 KIT (OKSA11272)

OKSA11272 25 Tests
EUR 717.6
Description: Description of target: FAM FLICAâ„¢ CASPASE-6 KIT;Species reactivity: ;Application: FC, IF;Assay info: ;Sensitivity:

FAM FLICAâ„¢ CASPASE-6 KIT (OKSA11273)

OKSA11273 100 Tests
EUR 1860
Description: Description of target: FAM FLICAâ„¢ CASPASE-6 KIT;Species reactivity: ;Application: FC, IF;Assay info: ;Sensitivity:

SR FLICA CASPASE-9 KIT

GWB-Q00475 25 TESTS Ask for price

SR FLICA CASPASE-9 KIT

GWB-Q00476 100 TESTS Ask for price

SR FLICA POLY CASPASE KIT

GWB-Q00477 25 TESTS Ask for price

SR FLICA POLY CASPASE KIT

GWB-Q00478 100 TESTS Ask for price

FAM FLICAâ„¢ CASPASE-8 KIT (OKSA11276)

OKSA11276 25 Tests
EUR 709.2
Description: Description of target: FAM FLICAâ„¢ CASPASE-8 KIT;Species reactivity: ;Application: FC, IF;Assay info: ;Sensitivity:

FAM FLICAâ„¢ CASPASE-8 KIT (OKSA11277)

OKSA11277 100 Tests
EUR 1828.8
Description: Description of target: FAM FLICAâ„¢ CASPASE-8 KIT;Species reactivity: ;Application: FC, IF;Assay info: ;Sensitivity:

FAM FLICAâ„¢ CASPASE-9 KIT (OKSA11278)

OKSA11278 25 Tests
EUR 709.2
Description: Description of target: FAM FLICAâ„¢ CASPASE-9 KIT;Species reactivity: ;Application: FC, IF;Assay info: ;Sensitivity:

FAM FLICAâ„¢ CASPASE-9 KIT (OKSA11279)

OKSA11279 100 Tests
EUR 1828.8
Description: Description of target: FAM FLICAâ„¢ CASPASE-9 KIT;Species reactivity: ;Application: FC, IF;Assay info: ;Sensitivity:

FAM FLICAâ„¢ CASPASE-2 KIT (OKSA11282)

OKSA11282 25 Tests
EUR 668.4
Description: Description of target: FAM FLICAâ„¢ CASPASE-2 KIT;Species reactivity: ;Application: FC, IF;Assay info: ;Sensitivity:

FAM FLICAâ„¢ POLY CASPASE KIT (OKSA11268)

OKSA11268 25 Tests
EUR 709.2
Description: Description of target: FAM FLICAâ„¢ POLY CASPASE KIT;Species reactivity: ;Application: FC, IF;Assay info: ;Sensitivity:

FAM FLICAâ„¢ POLY CASPASE KIT (OKSA11269)

OKSA11269 100 Tests
EUR 1828.8
Description: Description of target: FAM FLICAâ„¢ POLY CASPASE KIT;Species reactivity: ;Application: FC, IF;Assay info: ;Sensitivity:

OKSA11282-25T - FAM FLICAâ„¢ CASPASE-2 KIT

OKSA11282-25T 25Tests
EUR 429

SR-FLICA® Poly Caspase Assay Kit

916 25
EUR 216

SR-FLICA® Poly Caspase Assay Kit

917 100
EUR 615

OKSA11301-25T - SR FLICA™ CASPASE-9 KIT

OKSA11301-25T 25Tests
EUR 469

OKSA11302-100T - SR FLICA™ CASPASE-9 KIT

OKSA11302-100T 100Tests
EUR 1289

OKSA11280-25T - SR FLICA™ POLY CASPASE KIT

OKSA11280-25T 25Tests
EUR 489

OKSA11281-100T - SR FLICA™ POLY CASPASE KIT

OKSA11281-100T 100Tests
EUR 1249

Caspase 6 Assay Kit

20-abx299018
  • Ask for price
  • Ask for price
  • 100 tests
  • 25 tests

Caspase 6 Assay Kit, green

KF17204 25 Tests
EUR 364.8

Caspase 6 (CASP6) Assay Kit

abx299018-100g 100 µg Ask for price

Caspase 6 (CASP6) Assay Kit

abx299018-20g 20 µg
EUR 462.5

Caspase 6 (CASP6) Assay Kit

abx299018-50g 50 µg
EUR 1200

Caspase-6 Assay Kit (Colorimetric)

KTA3024-100T 100 T
EUR 289
Description: Caspase-6 Assay Kit (Colorimetric) provides a simple and convenient method for detecting the activity of caspase-6.

Caspase-6 Assay Kit (Colorimetric)

KTA3024-20T 20 T
EUR 89
Description: Caspase-6 Assay Kit (Colorimetric) provides a simple and convenient method for detecting the activity of caspase-6.

Caspase-6 Assay Kit (Colorimetric)

KTA3024-50T 50 T
EUR 159
Description: Caspase-6 Assay Kit (Colorimetric) provides a simple and convenient method for detecting the activity of caspase-6.

Caspase-6 Microplate Assay Kit

DLSM0197 100 Assays
EUR 245
Description: Detection and Quantification of Caspase-6 Activity.

Caspase-6 Fluorometric Assay Kit

K2014-100 100 assays
EUR 322
Description: Detects VEID-dependent caspase activity, highly-sensitive.

Caspase-6 Fluorometric Assay Kit

K2014-200 200 assays
EUR 492
Description: Detects VEID-dependent caspase activity, highly-sensitive.

Caspase-6 Fluorometric Assay Kit

K2014-25 25 assays
EUR 143
Description: Detects VEID-dependent caspase activity, highly-sensitive.

Caspase-6 Fluorometric Assay Kit

K2014-400 400 assays
EUR 789
Description: Detects VEID-dependent caspase activity, highly-sensitive.

Caspase-6 Colorimetric Assay Kit

K2015-100 100 assays
EUR 318
Description: Detects VEID-dependent caspase activity, highly-sensitive.

Caspase-6 Colorimetric Assay Kit

K2015-200 200 assays
EUR 469
Description: Detects VEID-dependent caspase activity, highly-sensitive.

Caspase-6 Colorimetric Assay Kit

K2015-25 25 assays
EUR 136
Description: Detects VEID-dependent caspase activity, highly-sensitive.

Caspase-6 Colorimetric Assay Kit

K2015-400 400 assays
EUR 799
Description: Detects VEID-dependent caspase activity, highly-sensitive.

Caspase-6 Fluorometric Assay Kit

K114-100 each
EUR 516

Caspase-6 Fluorometric Assay Kit

K114-200 each
EUR 738

Caspase-6 Fluorometric Assay Kit

K114-25 each
EUR 242.4

Caspase-6 Fluorometric Assay Kit

K114-400 each
EUR 1149.6

Caspase-6 Colorimetric Assay Kit

K115-100 each
EUR 542.4

Caspase-6 Colorimetric Assay Kit

K115-200 each
EUR 738

Caspase-6 Colorimetric Assay Kit

K115-25 each
EUR 242.4

Caspase-6 Colorimetric Assay Kit

K115-400 each
EUR 1149.6

Caspase 6 Fluorometric Assay Kit

55R-1276 25 assays
EUR 467
Description: Assay Kit for detection of Capase 6 activity in the research laboratory

Caspase 6 Colorimetric Assay Kit

55R-1277 25 assays
EUR 493
Description: Assay Kit for detection of Capase 6 activity in the research laboratory

Caspase-6 Fluorometric Assay Kit - 100 assays

GWB-AXR040 100 assays Ask for price

Caspase-6 Fluorometric Assay Kit - 200 assays

GWB-AXR041 200 assays Ask for price

Caspase-6 Fluorometric Assay Kit - 400 assays

GWB-AXR043 400 assays Ask for price

Caspase-6 Colorimetric Assay Kit - 100 assays

GWB-AXR044 100 assays Ask for price

Caspase-6 Colorimetric Assay Kit - 200 assays

GWB-AXR045 200 assays Ask for price

Caspase-6 Colorimetric Assay Kit - 400 assays

GWB-AXR047 400 assays Ask for price

Caspase-6 Fluorometric Assay Kit - 25 assays

GWB-AXR042 25 assays Ask for price

Caspase-6 Colorimetric Assay Kit - 25 assays

GWB-AXR046 25 assays Ask for price

Caspase 3 Assay Kit

20-abx294003
  • Ask for price
  • Ask for price
  • Ask for price
  • 100 tests
  • 20 tests
  • 50 tests

Caspase 1 Assay Kit

20-abx299019
  • Ask for price
  • Ask for price
  • 100 tests
  • 25 tests

Caspase 8 Assay Kit

20-abx299020
  • Ask for price
  • Ask for price
  • 100 tests
  • 25 tests

Caspase 9 Assay Kit

20-abx299021
  • Ask for price
  • Ask for price
  • 100 tests
  • 25 tests

Caspase 1 Assay Kit

abx299023-2550tests 25-50 tests
EUR 830.4

Caspase 2 Assay Kit

abx299026-25tests 25 tests
EUR 698.4

Caspase 10 Assay Kit

abx299027-100tests 100 tests
EUR 1562.4

Caspase 9 Assay Kit

20-abx299035
  • Ask for price
  • Ask for price
  • 100 tests
  • 25 tests

Caspase 12 Assay Kit

55R-1290 25 assays
EUR 553
Description: Assay Kit for detection of Capase 12 activity in the research laboratory

Caspase 6 (CASP6) Magnetic Luminex Assay Kit

LKU603118-96T 96T
EUR 966

Genorise® Caspase-6 Colorimetric Assay Kit

GR107023 100 tests
EUR 289

Genorise® Caspase-6 Fluorometric Assay Kit

GR107024 100 tests
EUR 289

Caspase 3/7 Assay Kit

20-abx299017
  • Ask for price
  • Ask for price
  • 100 tests
  • 25 tests

Caspase 3/7 Assay Kit

abx299024-2550tests 25-50 tests
EUR 830.4

Caspase 3/7 Assay Kit

20-abx299028
  • Ask for price
  • Ask for price
  • 100 tests
  • 25 tests

Caspase 3/7 Assay Kit

abx299017-100g 100 µg Ask for price

Caspase 3/7 Assay Kit

abx299017-20g 20 µg
EUR 462.5

Caspase 3/7 Assay Kit

abx299017-50g 50 µg
EUR 1200

Caspase 3/7 Assay Kit

abx299024-100g 100 µg Ask for price

Caspase 3/7 Assay Kit

abx299024-20g 20 µg
EUR 575

Caspase 3/7 Assay Kit

abx299024-50g 50 µg Ask for price

Caspase 3/7 Assay Kit

abx299028-100g 100 µg Ask for price

Caspase 3/7 Assay Kit

abx299028-20g 20 µg
EUR 450

Caspase 3/7 Assay Kit

abx299028-50g 50 µg
EUR 1050

Caspase 9 Assay Kit, red

KF17302 25 Tests
EUR 364.8

660 Caspase-1 Assay Kit

KF17362 25-50 Tests
EUR 418.8

Caspase 1 Assay Kit, green

KF17201 25 Tests
EUR 390

Caspase 2 Assay Kit, green

KF17202 25 Tests
EUR 364.8

Caspase 8 Assay Kit, green

KF17205 25 Tests
EUR 364.8

Caspase 9 Assay Kit, green

KF17206 25 Tests
EUR 364.8

Caspase 10 Assay Kit, green

KF17207 25 Tests
EUR 364.8

Caspase 13 Assay Kit, green

KF17208 25 Tests
EUR 364.8

SR FLICAâ„¢ CASPASE-9 KIT (OKSA11301)

OKSA11301 25 Tests
EUR 724.8
Description: Description of target: SR FLICAâ„¢ CASPASE-9 KIT;Species reactivity: ;Application: FC, IF;Assay info: ;Sensitivity:

SR FLICAâ„¢ CASPASE-9 KIT (OKSA11302)

OKSA11302 100 Tests
EUR 1884
Description: Description of target: SR FLICAâ„¢ CASPASE-9 KIT;Species reactivity: ;Application: FC, IF;Assay info: ;Sensitivity:

Caspase 3 / Caspase 7 (CASP3/CASP7) Assay Kit

abx294003-100l 100 µl
EUR 350

Caspase 6 Activity Assay Kit(Colorimetric Method)

E-CK-A386-each each Ask for price
Description: Apoptosis/Caspase

Caspase 1 (CASP1) Assay Kit

abx299019-100g 100 µg Ask for price

Caspase 1 (CASP1) Assay Kit

abx299019-20g 20 µg
EUR 512.5

Caspase 1 (CASP1) Assay Kit

abx299019-50g 50 µg
EUR 1262.5

Caspase 8 (CASP8) Assay Kit

abx299020-100g 100 µg Ask for price

Caspase 8 (CASP8) Assay Kit

abx299020-20g 20 µg
EUR 462.5

Caspase 8 (CASP8) Assay Kit

abx299020-50g 50 µg
EUR 1200

Caspase 9 (CASP9) Assay Kit

abx299021-100g 100 µg Ask for price

Caspase 9 (CASP9) Assay Kit

abx299021-20g 20 µg
EUR 462.5

Caspase 9 (CASP9) Assay Kit

abx299021-50g 50 µg
EUR 1200

Caspase 1 (CASP1) Assay Kit

abx299023-100g 100 µg Ask for price

Caspase 1 (CASP1) Assay Kit

abx299023-20g 20 µg
EUR 575

Caspase 1 (CASP1) Assay Kit

abx299023-50g 50 µg Ask for price

Caspase 2 (CASP2) Assay Kit

abx299026-100g 100 µg Ask for price

Caspase 2 (CASP2) Assay Kit

abx299026-20g 20 µg
EUR 462.5

Caspase 2 (CASP2) Assay Kit

abx299026-50g 50 µg Ask for price

Caspase 9 (CASP9) Assay Kit

abx299035-100g 100 µg Ask for price

Caspase 9 (CASP9) Assay Kit

abx299035-20g 20 µg
EUR 437.5

Caspase 9 (CASP9) Assay Kit

abx299035-50g 50 µg
EUR 1200

QuantiFluo Caspase-3 Assay Kit

DCS3-100 100
EUR 289

Caspase 10 (CASP10) Assay Kit

abx299027-100g 100 µg Ask for price

Caspase 10 (CASP10) Assay Kit

abx299027-20g 20 µg
EUR 1200

Caspase 10 (CASP10) Assay Kit

abx299027-50g 50 µg Ask for price

SR FLICAâ„¢ POLY CASPASE KIT (OKSA11280)

OKSA11280 25 Tests
EUR 756
Description: Description of target: SR FLICAâ„¢ POLY CASPASE KIT;Species reactivity: ;Application: FC, IF;Assay info: ;Sensitivity:

SR FLICAâ„¢ POLY CASPASE KIT (OKSA11281)

OKSA11281 100 Tests
EUR 1828.8
Description: Description of target: SR FLICAâ„¢ POLY CASPASE KIT;Species reactivity: ;Application: FC, IF;Assay info: ;Sensitivity:

Caspase-1 Activity Assay Kit

AR4003-100Assays 100 Assays
EUR 616.8

Caspase-1 Activity Assay Kit

AR4003-25Assays 25 Assays
EUR 283.2

Caspase-1 Activity Assay Kit

AR4003-50Assays 50 Assays
EUR 408

Caspase-2 Activity Assay Kit

AR4004-100Assays 100 Assays
EUR 616.8

Caspase-2 Activity Assay Kit

AR4004-25Assays 25 Assays
EUR 283.2

Caspase-2 Activity Assay Kit

AR4004-50Assays 50 Assays
EUR 408

Caspase-1 Assay Kit (Colorimetric)

KTA3020-100T 100 T
EUR 289
Description: Caspase-1 Assay Kit (Colorimetric) provides a simple and convenient method for detecting the activity of caspase-1.

A Preliminary Study on the Ability of the Trypsin-Like Peptidase Activity Assay Kit to Detect Periodontitis

 This study aimed to explore whether the Trypsin-Like Peptidase Activity Assay Kit (TLP-AA-Kit), which measures the activity of N-benzoyl-dl-arginine peptidase (trypsin-like peptidase), can be used as a reliable tool for periodontitis detection in population-based surveillance. In total, 105 individuals underwent a full-mouth periodontal examination and provided tongue swabs as specimens for further analyses. The results of the TLP-AA-Kit were scored between 1 and 5; higher scores indicated higher trypsin concentrations. Receiver operating characteristic analyses were used to evaluate the predictive validity of the TLP-AA-Kit, where the periodontitis case definition provided by the Centers for Disease Control/American Academy of Periodontology served as the reference.

Severe and moderate periodontitis were identified in 4.8% and 16.2% of the study population, respectively. The TLP-AA-Kit showed high diagnostic accuracy for severe periodontitis, with an area under the curve of 0.93 (95% confidence interval = 0.88-0.99). However, the diagnostic accuracy of the TLP-AA-Kit for moderate/severe periodontitis was not reliable. While further studies are necessary to validate our results, the results provided herein highlight the potential of the TLP-AA-Kit as a useful tool for the detection of periodontitis, particularly in severe cases, for population-based surveillance.

Comparative Performance of SARS-CoV-2 Detection Assays using Seven Different Primer/Probe Sets and One Assay Kit 

More than 100,000 people worldwide are known to have been infected with SARS-CoV-2 beginning in December 2019. The virus has now spread to over 93 countries including the United States, with the largest cluster of US cases to date in the Seattle metropolitan area in Washington. Given the rapid increase in the number of local cases, the availability of accurate, high-throughput SARS-CoV-2 testing is vital to efforts to manage the current public health crisis. In the course of optimizing SARS-CoV-2 testing performed by the University of Washington Clinical Virology Lab (UW Virology Lab), we tested assays using seven different primer/probe sets and one assay kit.

We found that the most sensitive assays were those the used the E-gene primer/probe set described by Corman et al. (Eurosurveillance 25(3), 2020, https://doi.org/10.2807/1560-7917.ES.2020.25.3.2000045) and the N2 set described by the CDC (Division of Viral Diseases, Centers for Disease Control and Prevention, 2020, https://www.cdc.gov/coronavirus/2019-ncov/downloads/rt-pcr-panel-primer-probes.pdf). All assays tested were found to be highly specific for SARS-CoV-2, with no cross-reactivity with other respiratory viruses observed in our analyses regardless of the primer/probe set or kit used. These results will provide invaluable information to other clinical laboratories who are actively developing SARS-CoV-2 testing protocols at a time when increased testing capacity is urgently needed worldwide.

Comparative Performance of SARS-CoV-2 Detection Assays using Seven Different Primer/Probe Sets and One Assay Kit.

 Nearly 400,000 people worldwide are known to have been infected with SARS-CoV-2 beginning in December 2019. The virus has now spread to over 168 countries including the United States, where the first cluster of cases was observed in the Seattle metropolitan area in Washington. Given the rapid increase in the number of cases in many localities, the availability of accurate, high-throughput SARS-CoV-2 testing is vital to efforts to manage the current public health crisis.

In the course of optimizing SARS-CoV-2 testing performed by the University of Washington Clinical Virology Lab (UW Virology Lab), we evaluated assays using seven different primer/probe sets and one assay kit. We found that the most sensitive assays were those that used the E-gene primer/probe set described by Corman et al. (Eurosurveillance 25 (3), 2020, https://doi.org/10.2807/1560-7917.ES.2020.25.3.2000045) and the N2 set developed by the CDC (Division of Viral Diseases, Centers for Disease Control and Prevention, 2020,

All assays tested were found to be highly specific for SARS-CoV-2, with no cross-reactivity with other respiratory viruses observed in our analyses regardless of the primer/probe set or kit used. These results will provide valuable information to other clinical laboratories who are actively developing SARS-CoV-2 testing protocols at a time when increased testing capacity is urgently needed worldwide.

Development and validation of a rapid kit for authenticity of murine meat in meat products with a species-specific PCR assay.

 Adulteration of meat products with murine meat poses a huge threat to consumer health and leads to serious disruption in food markets. Species authentication of murine meat is still technically challenging. We, therefore, developed a species-specific PCR kit consisting of murine meat DNA extraction, PCR reaction and identifying systems. We designed novel universal primers targeting highly conserved region on mitochondrial cytochrome b gene (cyt b) from four murines (lab rats, lab mice, wild rat and wild mice), as well as specific primers for meat from four widely consumed animal species, cattle, sheep, duck and donkey. Simultaneously, pasmid inserted specific cyt b fragment was cloned and used as the internal positve control in the kit. The kit parameters of specificity, sensitivity, stability and validity were determined using mimic counterfeiting meatball.

The specificity of the DNA detection kit was 100% in authentication of the four fraudulent meats of cattle, sheep, duck and donkey mixed murine meat. The minimum detection limit of the sample DNA was 0.1 μg. The kit, which had freeze-thawed up to 20 times and stored for 1 year, also was powerful in detecting an amount of 0.1 mg in artificial counterfeited cattle, sheep, duck and donkey meat products. The murine-species DNA detection kit proposed in this study has proved to be a simple, accurate and effective assay, and can be applied to the identification of murine meat traces in common edible meat, to ensure the realisable implementation of meat product market supervision.

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