Evaluation of ” Caterina assay”: An Alternative Tool

oxfordgenomesciences

Evaluation of ” Caterina assay“: An Alternative Tool to the Commercialized Kits Used for Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Identification

Here we describe the first molecular test developed in the early stage of the pandemic to diagnose the first cases of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection in Sardinian patients in February-March 2020, when diagnostic certified methodology had not yet been adopted by clinical microbiology laboratories. The “Caterina assay” is a SYBR®Green real-time reverse-transcription polymerase chain reaction (rRT-PCR), designed to detect the nucleocapsid phosphoprotein (N) gene that exhibits high discriminative variation RNA sequence among bat and human coronaviruses.

The molecular method was applied to detect SARS-CoV-2 in nasal swabs collected from 2110 suspected cases. The study article describes the first molecular test developed in the early stage of the declared pandemic to identify the coronavirus disease 2019 (COVID-19) in Sardinian patients in February-March 2020, when a diagnostic certified methodology had not yet been adopted by clinical microbiology laboratories.

The assay presented high specificity and sensitivity (with a detection limit ≥50 viral genomes/μL). No false-positives were detected, as confirmed by the comparison with two certified commercial kits. Although other validated molecular methods are currently in use, the Caterina assay still represents a valid and low-cost detection procedure that could be applied in countries with limited economic resources.

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oxfordgenomesciences
FAM-Spacer-Phe-CMK FLISP™ Assay Kit
KF17323 100 Tests
EUR 695
FAM-Leu-CMK FLISP™ Assay Kit
KF17317 100 Tests
EUR 695
FAM-Phe-CMK FLISP™ Assay Kit
KF17314 25 Tests
EUR 291
FAM-Phe-CMK FLISP™ Assay Kit
KF17315 100 Tests
EUR 695
FAM-Lue-CMK FLISP™ Assay Kit
KF17316 25 Tests
EUR 291
SR-101-Leu-CMK FLISP™ Assay Kit
KF17321 100 Tests
EUR 695
FAM-Leu-CMK Serine Protease Assay Kit
abx299011-25tests 25 tests
EUR 566
  • Shipped within 1 week.
SR-101-Phe-CMK FLISP™ Assay Kit
KF17318 25 Tests
EUR 291
SR-101-Phe-CMK FLISP™ Assay Kit
KF17319 100 Tests
EUR 695
FAM-Phe-CMK Serine Protease Assay Kit
abx299010-25tests 25 tests
EUR 566
  • Shipped within 1 week.
FAM-Leu-DAP Serine Protease Assay Kit
abx299012-25tests 25 tests
EUR 566
  • Shipped within 1 week.
OPTIONAL GREEN FILTER (572NM)
GDBL-GREEN 1/pk
EUR 1052
Description: Lab Equipment; Axygen Branded EQ
Polycaspase Assay Kit, green
KF17200 25 Tests
EUR 304
Malachite Green Phosphate Assay Kit
abx298878-100Assays 100 Assays
EUR 378
  • Shipped within 5-10 working days.
Caspase 1 Assay Kit, green
KF17201 25 Tests
EUR 325
Caspase 2 Assay Kit, green
KF17202 25 Tests
EUR 304
Caspase 6 Assay Kit, green
KF17204 25 Tests
EUR 304
Caspase 8 Assay Kit, green
KF17205 25 Tests
EUR 304
Caspase 9 Assay Kit, green
KF17206 25 Tests
EUR 304
Caspase 10 Assay Kit, green
KF17207 25 Tests
EUR 304
Caspase 13 Assay Kit, green
KF17208 25 Tests
EUR 304
Malachite Green Phosphate Assay Kit
POMG-25H 2500
EUR 275
Description: High-throµghput phosphate assay using malachite green method at 620nm. Kit size: 2500 tests. Detection limit: 0.02 µM. Shelf life: 12 months. Shipping: ambient temp; storage: 4°C.
Malachite Green Phosphate Assay Kit
Z5030012 2,500 assays
EUR 342
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.
FAM-Phe-DAP Serine Protease Assay Kit
abx299013-25tests 25 tests
EUR 582
  • Shipped within 1 week.
FAM-cAMP PDE IV substrate *Green Fluorescence*
13602 0.5 umol
EUR 306
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200
FAM-cGMP PDE V substrate *Green Fluorescence*
13604 0.5 umol
EUR 306
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200
Caspase 3 & 7 Assay Kit, green
KF17203 25 Tests
EUR 304
EZCell? Phagocytosis Assay Kit (Green Zymosan)
K397-100
EUR 430
EZCell? Phagocytosis Assay Kit (Green E. coli)
K963-100
EUR 430
OxiSelect Intracellular ROS Assay Kit (Green Fluorescence)
STA-342 96 assays
EUR 635
Description: The OxiSelect ROS Assay Kit is a cell-based assay for measuring hydroxyl, peroxyl, and other reactive oxygen species activity within a cell. The assay employs the cell-permeable fluorogenic probe DCFH-DA, which diffuses into cells and is deacetylcated by cellular esterases into the non-fluorescent DCFH (Figure 1). In the presence of ROS, DCFH is rapidly oxidized to highly fluorescent DCF. Fluorescence is read on a standard fluorometric plate reader.
OxiSelect Intracellular ROS Assay Kit (Green Fluorescence)
STA-342-5 5 x 96 assays
EUR 2549
Description: The OxiSelect ROS Assay Kit is a cell-based assay for measuring hydroxyl, peroxyl, and other reactive oxygen species activity within a cell. The assay employs the cell-permeable fluorogenic probe DCFH-DA, which diffuses into cells and is deacetylcated by cellular esterases into the non-fluorescent DCFH (Figure 1). In the presence of ROS, DCFH is rapidly oxidized to highly fluorescent DCF. Fluorescence is read on a standard fluorometric plate reader.
OxiSelect Cellular Antioxidant Assay Kit (Green Fluorescence)
STA-349 192 assays
EUR 537
Description: The OxiSelect Cellular Antioxidant Assay Kit is a cell-based assay for measuring the activity of an exogenous antioxidant compound within adherent cells.  Cells are first cultured in a 96-well black fluorescence cell culture plate until confluent. Then the cells are pre-incubated with a cell-permeable DCFH-DA fluorescence probe dye and the bioflavonoid Quercetin, or the antioxidant sample being tested.  After a brief incubation, the cells are washed, and the reaction started by adding the Free Radical Initiator.  The Free Radical Initiator creates free radicals that convert the probe to highly fluorescent DCF.  The Quercetin inhibits the formation of free radicals, and thus DCF formation, in a concentration dependent manner.
Amplite™ Fluorimetric Acetylcholinesterase Assay Kit *Green Fluorescence*
11401 200 Tests
EUR 219
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12171501
Magainin Spacer Peptide
5-01496 4 x 1mg Ask for price
spacer 0.8mm (2)
EVS3100-SP-0.8 ea
EUR 40
spacer 1.5mm (2)
EVS3100-SP-1.5 ea
EUR 40
spacer 0.8mm (2)
EVS3200-SP-0.8 ea
EUR 48
spacer 1.5mm (2)
EVS3200-SP-1.5 ea
EUR 48
spacer 0.8mm (2)
EVS3300-SP-0.8 ea
EUR 48
spacer 1.5mm (2)
EVS3300-SP-1.5 ea
EUR 48
CMK
A3319-10 10 mg
EUR 502
Description: CMK is a RSK2 kinase inhibitor.
CMK
A3319-25 25 mg
EUR 701
Description: CMK is a RSK2 kinase inhibitor.
CMK
A3319-5 5 mg
EUR 321
Description: CMK is a RSK2 kinase inhibitor.
CMK
A3319-5.1 10 mM (in 1mL DMSO)
EUR 264
Description: CMK is a RSK2 kinase inhibitor.
CMK
HY-52101 2mg
EUR 187
Cell Meter™ Cell Viability Assay Kit *Green Fluorescence*
22786 500 Tests
EUR 219
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200
Cell Meter™ TUNEL Apoptosis Assay Kit *Green Fluorescence*
22849 50 Tests
EUR 306
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200
Amplite™ MMP-3 Activity Assay Kit *Green Fluorescence*
13512 100 Tests
EUR 306
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200
Amplite™ Fluorimetric HDAC Activity Assay Kit *Green Fluorescence*
13601 200 Tests
EUR 219
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200
Amplite™ Fluorimetric Alkaline Phosphatase Assay Kit *Green Fluorescence*
11953 500 Tests
EUR 219
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12171501
Amplite™ Fluorimetric Beta-Galactosidase Assay Kit *Green Fluorescence*
12601 500 Tests
EUR 306
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200
EZClick? Myristoylated Protein Assay Kit (FACS/Microscopy), Green Fluorescence
K497-100
EUR 490
EZClick? Palmitoylated Protein Assay Kit (FACS/Microscopy), Green Fluorescence
K452-100
EUR 490
EZClick? Stearoylated Protein Assay Kit (FACS/Microscopy), Green Fluorescence
K453-100
EUR 490
OxiSelect Intracellular ROS Assay Kit (Green Fluorescence), Trial Size
STA-342-T 20 assays
EUR 345
Description: The OxiSelect ROS Assay Kit is a cell-based assay for measuring hydroxyl, peroxyl, and other reactive oxygen species activity within a cell. The assay employs the cell-permeable fluorogenic probe DCFH-DA, which diffuses into cells and is deacetylcated by cellular esterases into the non-fluorescent DCFH (Figure 1). In the presence of ROS, DCFH is rapidly oxidized to highly fluorescent DCF. Fluorescence is read on a standard fluorometric plate reader.
OxiSelect In Vitro ROS/RNS Assay Kit (Green Fluorescence)
STA-347 96 assays
EUR 612
Description: The OxiSelect In Vitro ROS/RNS Assay provides a sensitive method to detect total reactive oxygen species (ROS) plus reactive nitrogen species (RNS) in a wide variety of sample types. This assay employs a proprietary fluorogenic probe, DCFH-DiOxyQ; the probe is primed with a dequenching reagent to the highly reactive DCFH form. In the presence of ROS and RNS, the DCFH is rapidly oxidized to the highly fluorescent DCF.
OxiSelect In Vitro ROS/RNS Assay Kit (Green Fluorescence)
STA-347-5 5 x 96 assays
EUR 2428
Description: The OxiSelect In Vitro ROS/RNS Assay provides a sensitive method to detect total reactive oxygen species (ROS) plus reactive nitrogen species (RNS) in a wide variety of sample types. This assay employs a proprietary fluorogenic probe, DCFH-DiOxyQ; the probe is primed with a dequenching reagent to the highly reactive DCFH form. In the presence of ROS and RNS, the DCFH is rapidly oxidized to the highly fluorescent DCF.
Cell Navigator™ Fluorimetric Lipid Droplet Assay Kit *Green Fluorescence*
22730 200 Tests
EUR 219
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200
Amplite™ Fluorimetric Proteasome 20S Activity Assay Kit *Green Fluorescence*
13456 100 tests
EUR 306
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200
Amplite™ Universal Fluorimetric Protease Activity Assay Kit *Green Fluorescence*
13500 500 Tests
EUR 219
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200
Amplite™ Fluorimetric Caspase 3/7 Assay Kit *Green Fluorescence*
13503 500 Tests
EUR 306
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200
Amplite™ Universal Fluorimetric MMP Activity Assay Kit *Green Fluorescence*
13510 100 Tests
EUR 306
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200
Cell Meter™ Fluorimetric Intracellular Peroxynitrite Assay Kit *Green Fluorescence*
16315 100 Tests
EUR 350
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200
Amplite™ Fluorimetric Total Thiol Quantitation Assay Kit *Green Fluorescence*
5524 200 Tests
EUR 219
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200
EZClick? Global Protein Synthesis Assay Kit (FACS/Microscopy), Green Fluorescence
K459-100
EUR 550
cmk Antibody
1-CSB-PA363865HA01EVN
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
  • Form: Liquid
  • Buffer: Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 >95%, Protein G purified
Description: A polyclonal antibody against cmk. Recognizes cmk from Escherichia coli. This antibody is Unconjugated. Tested in the following application: ELISA
5-FAM
20-abx095073
  • EUR 523.00
  • EUR 370.00
  • EUR 1274.00
  • 1 g
  • 500 mg
  • 5 g
  • Shipped within 5-10 working days.
6-FAM
HY-66021 500mg
EUR 312
5-FAM
HY-66022 10mM/1mL
EUR 126
Cell Meter™ Caspase 8 Activity Apoptosis Assay Kit *Green Fluorescence*
22798 200 Tests
EUR 219
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200
Cell Meter™ Caspase 9 Activity Apoptosis Assay Kit *Green Fluorescence*
22799 200 Tests
EUR 306
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200
Cell Meter™ Intracellular Fluorimetric Hydrogen Peroxide Assay Kit *Green Fluorescence*
11503 200 Tests
EUR 263
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12171501
Cell Meter™ Fluorimetric Mitochondrial Superoxide Activity Assay Kit *Green Fluorescence*
16060 200 Tests
EUR 306
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200
Amplite™ Rapid Fluorimetric Total Thiol Quantitation Assay Kit *Green Fluorescence*
5528 200 Tests
EUR 219
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200
EZClick? O-GalNAc Modified Glycoprotein Assay Kit (FACS/Microscopy, Green Fluorescence)
K560-100
EUR 490
EZClickTM Fucose (FucAz) Modified Glycoprotein Assay Kit (FACS/Microscopy, Green Fluorescence)
K592-100
EUR 490
EZClick? O-GlcNAc Modified Glycoprotein Assay Kit (FACS/Microscopy, Green Fluorescence)
K714-100
EUR 512
OxiSelect In Vitro ROS/RNS Assay Kit (Green Fluorescence), Trial Size
STA-347-T 20 assays
EUR 334
Description: The OxiSelect In Vitro ROS/RNS Assay provides a sensitive method to detect total reactive oxygen species (ROS) plus reactive nitrogen species (RNS) in a wide variety of sample types. This assay employs a proprietary fluorogenic probe, DCFH-DiOxyQ; the probe is primed with a dequenching reagent to the highly reactive DCFH form. In the presence of ROS and RNS, the DCFH is rapidly oxidized to the highly fluorescent DCF.
Branched Chain Amino Acid (Leu/Ile/Val) Colorimetric Assay Kit
K564-100
EUR 539
Branched Chain Amino Acid (Leu/Ile/Val) Colorimetric Assay Kit
K2085-100 100 assays
EUR 529
Cell Meter™ Live Cell Caspase 1 Binding Assay Kit *Green Fluorescence*
20108 25 Tests
EUR 306
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200
Cell Meter™ Live Cell Caspase 2 Binding Assay Kit *Green Fluorescence*
20111 25 Tests
EUR 306
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200
Cell Meter™ Live Cell Caspase 8 Binding Assay Kit *Green Fluorescence*
20115 25 Tests
EUR 306
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200
Cell Meter™ Live Cell Caspase 9 Binding Assay Kit *Green Fluorescence*
20117 25 Tests
EUR 306
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200
Cell Meter™ Live Cell Caspase 13 Binding Assay Kit *Green Fluorescence*
20125 25 Tests
EUR 306
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200
Live or Dead™ Cell Viability Assay Kit *Green/Red Dual Fluorescence*
22789 200 Tests
EUR 219
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200
Cell Meter™ Caspase 3/7 Activity Apoptosis Assay Kit *Green Fluorescence*
22796 200 Tests
EUR 219
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200
Cell Meter™ Fluorimetric Intracellular Total ROS Activity Assay Kit*Green Fluorescence*
22900 200 Tests
EUR 219
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200
EZClick? Sialic Acid (ManAz) Modified Glycoprotein Assay Kit (FACS/Microscopy, Green Fluorescence)
K441-100
EUR 490
Decanoyl-RVKR-CMK
B5437-.5 500 µg
EUR 154
Decanoyl-RVKR-CMK
B5437-1 1 mg
EUR 228
Decanoyl-RVKR-CMK
B5437-5 5 mg
EUR 885
Ac-DEVD-CMK
C5528-1 1 mg
EUR 139
Description: Ac-DEVD-CMK is a cell-permeable and irreversible caspase-3 inhibitor [1][2][3]. Apoptosis is a process of programmed cell death that occurs in multicellular organisms.
Ac-DEVD-CMK
C5528-5 5 mg
EUR 457
Description: Ac-DEVD-CMK is a cell-permeable and irreversible caspase-3 inhibitor [1][2][3]. Apoptosis is a process of programmed cell death that occurs in multicellular organisms.
cmk Polyclonal Antibody
A50845 100 µg
EUR 570.55
Description: The best epigenetics products
FAM-LETD-FMK
13416 100 ug
EUR 263
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200
5-FAM Alkyne
951 100 mg
EUR 702
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12171501
6-FAM Azide
955 100 mg
EUR 702
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12171501

Evaluation of a new semi-automated Hydragel 11 von Willebrand factor multimers assay kit for routine use

Background: Accurate diagnosis and classification of von Willebrand disease (VWD) are essential for optimal management. The von Willebrand factor multimers analysis (VWF:MM) is an integral part of the diagnostic process in the phenotypic classification, especially in discrepant cases. The aim of this study was to evaluate the performance of a new Hydragel 11VWF multimer assay (H11VW).

Methods: Analytical performance characteristics such as repeatability (intra-assay variability, in gel between track variation), reproducibility (inter-assay variability, between gel variation), sensitivity, EQA performance and differences between two commercially available VWF:MM kits (H5VW and H11VW) were analysed in healthy volunteers’ plasmas using in-house prepared reference plasma.

Results: Repeatability and reproducibility results of H11VW demonstrated acceptable and equivalent performance with previously verified H5VW. Participation in EQA was successful. No statistically significant difference was detected between H5VW and H11VW kits for different fractions of multimers: LMWM p=0.807; IMWM p=0.183; HMWM p=0.774.

Conclusions: H11VW demonstrated acceptable analytical performance characteristics. H11VW kit conveniently offers a more significant number of samples on a single gel. H5VW and H11VW kits can be used in daily practice interchangeably.

Developmental validation of VeriFiler™ Plus PCR Amplification Kit: A 6-dye multiplex assay designed for casework samples

The VeriFiler™ Plus PCR Amplification Kit is a 6-dye multiplex assay that simultaneously amplifies a set of 23 autosomal markers (D3S1358, vWA, D16S539, CSF1PO, D6S1043, D8S1179, D21S11, D18S51, D5S818, D2S441, D19S433, FGA, D10S1248, D22S1045, D1S1656, D13S317, D7S820, Penta E, Penta D, TH01, D12S391, D2S1338, and TPOX), a quality indicator system, and two sex-identification markers. Combined, the markers satisfy the requirements of the Chinese National autosomal DNA database as well as expanded CODIS (Combined DNA Index System).

The VeriFiler Plus kit was developed with an improved Master Mix which incorporates the brighter TED™ dye, and accommodates a higher sample loading volume thus allowing for increased sensitivity and enabling maximum information recovery from challenging casework samples including touch, degraded, and inhibited samples.

Here, we report the results of the developmental validation study which followed the SWGDAM (Scientific Working Group on DNA Analysis Methods) guidelines and includes data for PCR-based studies, sensitivity, species specificity, stability, precision, reproducibility and repeatability, concordance, stutter, DNA mixtures, and performance on mock casework samples. The results validate the multiplex design as well as demonstrate the kit’s robustness, reliability, and suitability as an assay for human identification with casework DNA samples.

Performance of Saliva Samples for COVID-19 Diagnosis by Using the Allplex TM 2019-nCoV Assay Kit

Background: Although the nasopharyngeal swab (NPS) is the reference sampling method for the detection of SARS-Cov-2, it is not always possible to collect NPS in some patients. Saliva represents an interesting sampling method because it is less invasive and more convenient in patients with nasal or pharyngeal lesions.

Objective: To compare the RT-qPCR test performances of saliva samples with nasal mid-turbinate swab (NMTS) and NPS samples in a cohort of ambulatory patients suspected of having COVID-19.

Study Design: For each of the 112 enrolled patients, NPS, NMTS, and saliva samples were collected and tested for SARS-Cov-2 detection using three different target genes (RdRP, N and E genes) by RT-qPCR.

Results: Among the positive samples (56/112), saliva samples showed a lower percentage of SARS-Cov-2 detection compared to NPS samples, (85.7 vs. 96.4%), while still a lower percentage was observed for NMTS samples (78.6%). In average, saliva samples showed higher Ct values for all tested target genes, compared to those from NPS and NMTS samples.

Conclusions: By using the AllplexTM 2019-nCoV Assay Kit, saliva samples showed lower sensitivity for SARS CoV-2 compared to NPS samples; however, the not detected cases had lower viral burden in NPS samples (CT values >33) representing an interesting alternative sampling method in patients in which it is not possible to take a NPS sample.

Application of Lithium Assay kit LS for quantification of lithium in whole blood and urine

A commercially available kit for the quantitation of lithium, the Lithium Assay kit LS, was originally developed to measure lithium in serum or plasma using a conventional microplate reader. We investigated whether use of the kit could be extended to quantify lithium in whole blood and urine samples collected at autopsy.

The calibration curve for whole blood showed good linearity ranging from 0.5 to 20 µg/mL with a coefficient of determination of 0.998 when samples were pretreated with methanol followed by acetonitrile. Moreover, for urine, we obtained excellent linearity with a coefficient of determination of 0.999 without any pretreatment.

The accuracies and precisions were 106.3-174.7% and 1.9-18.1% for whole blood and 83.3-118.8% and 5.7-33.8% for urine. The values in the lower concentration range (0.5-1 µg/mL) were not satisfactory, whereas those in the higher range (2-20 µg/mL) were acceptable. The Lithium Assay kit LS was successfully applied to the measurement of lithium in whole blood and urine samples collected at autopsies. This method appears to be useful for forensic toxicological investigations because of its simplicity and speed.

SR FLICAâ„¢ POLY CASPASE KIT (OKSA11280)

OKSA11280 25 Tests
EUR 630
Description: Description of target: SR FLICAâ„¢ POLY CASPASE KIT;Species reactivity: ;Application: FC, IF;Assay info: ;Sensitivity:

SR FLICAâ„¢ POLY CASPASE KIT (OKSA11281)

OKSA11281 100 Tests
EUR 1524
Description: Description of target: SR FLICAâ„¢ POLY CASPASE KIT;Species reactivity: ;Application: FC, IF;Assay info: ;Sensitivity:

FAM FLICA CASPASE-1 KIT (OKSA11274)

OKSA11274 25 Tests
EUR 591
Description: Description of target: FAM FLICAâ„¢ CASPASE-1 KIT;Species reactivity: ;Application: FC, IF;Assay info: ;Sensitivity:

FAM FLICA CASPASE-1 KIT (OKSA11275)

OKSA11275 100 Tests
EUR 1523
Description: Description of target: The FAM FLICATM Capase 1 kit uses a target sequence (YVAD) sandwiched between a green fluorescent label, FAM, and FMK to make a quick and flexible method to analyze active capases in apoptotic cells. These kits measure apoptosis by detecting active caspases in whole, living cells. They don't work by using antibodies or an ELISA. Instead, their methodology is based a unique cell-permeable and non-cytotoxic reagent called Fluorochrome Inhibitor of Caspases. These kits are suitable for cells in suspension, adherent cells, thin tissue sections (but not fixed or paraffin-embedded cells) from many species including mammalian, insect, and yeast. Different cell types, e.g. HeLa, primary neurons, macrophages and lymphocytes have also been successfully studied with these kits. ;Species reactivity: Human;Application: FC, IF;Assay info: ;Sensitivity:

FAM FLICA CASPASE-2 KIT (OKSA11283)

OKSA11283 100 Tests
EUR 1328
Description: Description of target: FAM FLICAâ„¢ CASPASE-2 KIT;Species reactivity: ;Application: FC, IF;Assay info: ;Sensitivity:

FAM FLICA CASPASE-10 KIT (OKSA11285)

OKSA11285 100 Tests
EUR 1563
Description: Description of target: FAM FLICAâ„¢ CASPASE-10 KIT;Species reactivity: ;Application: FC, IF;Assay info: ;Sensitivity:

FAM FLICA CASPASE-3/7 KIT (OKSA11271)

OKSA11271 100 Tests
EUR 1524
Description: Description of target: The FAM FLICA™ Caspase 3 & 7 kit uses a quick and easy method to analyze active caspases in apoptotic cells.;Species reactivity: ;Application: FC, IF;Assay info: ;Sensitivity:

SR FLICAâ„¢ CASPASE-9 KIT (OKSA11301)

OKSA11301 25 Tests
EUR 604
Description: Description of target: SR FLICAâ„¢ CASPASE-9 KIT;Species reactivity: ;Application: FC, IF;Assay info: ;Sensitivity:

SR FLICAâ„¢ CASPASE-9 KIT (OKSA11302)

OKSA11302 100 Tests
EUR 1570
Description: Description of target: SR FLICAâ„¢ CASPASE-9 KIT;Species reactivity: ;Application: FC, IF;Assay info: ;Sensitivity:

Caspase 12 Assay Kit

55R-1290 25 assays
EUR 328
Description: Assay Kit for detection of Capase 12 activity in the research laboratory

Caspase 3 Assay Kit

20-abx294003
  • EUR 1255.00
  • EUR 457.00
  • EUR 770.00
  • 100 tests
  • 20 tests
  • 50 tests
  • Shipped within 5-12 working days.

Caspase 6 Assay Kit

20-abx299018
  • EUR 1302.00
  • EUR 582.00
  • 100 tests
  • 25 tests
  • Shipped within 1 week.

Caspase 1 Assay Kit

20-abx299019
  • EUR 1365.00
  • EUR 629.00
  • 100 tests
  • 25 tests
  • Shipped within 1 week.

Caspase 8 Assay Kit

20-abx299020
  • EUR 1302.00
  • EUR 582.00
  • 100 tests
  • 25 tests
  • Shipped within 1 week.

Caspase 9 Assay Kit

20-abx299021
  • EUR 1302.00
  • EUR 582.00
  • 100 tests
  • 25 tests
  • Shipped within 1 week.

Caspase 1 Assay Kit

abx299023-2550tests 25-50 tests
EUR 692
  • Shipped within 1 week.

Caspase 2 Assay Kit

abx299026-25tests 25 tests
EUR 582
  • Shipped within 1 week.

Caspase 10 Assay Kit

abx299027-100tests 100 tests
EUR 1302
  • Shipped within 1 week.

Caspase 9 Assay Kit

20-abx299035
  • EUR 1302.00
  • EUR 582.00
  • 100 tests
  • 25 tests
  • Shipped within 1 week.

Glycerol Cell-Based Assay Kit

K977-100
EUR 490

FAM FLICAâ„¢ POLY CASPASE KIT (OKSA11268)

OKSA11268 25 Tests
EUR 591
Description: Description of target: FAM FLICAâ„¢ POLY CASPASE KIT;Species reactivity: ;Application: FC, IF;Assay info: ;Sensitivity:

FAM FLICAâ„¢ POLY CASPASE KIT (OKSA11269)

OKSA11269 100 Tests
EUR 1524
Description: Description of target: FAM FLICAâ„¢ POLY CASPASE KIT;Species reactivity: ;Application: FC, IF;Assay info: ;Sensitivity:

Caspase 3 Fluorometric Assay Kit

55R-1269 25 assays
EUR 277
Description: Assay Kit for detection of Capase 3 activity in the research laboratory

Caspase 3 Colorimetric Assay Kit

55R-1270 25 assays
EUR 277
Description: Assay Kit for detection of Capase 3 activity in the research laboratory

Caspase 1 Fluorometric Assay Kit

55R-1272 25 assays
EUR 277
Description: Assay Kit for detection of Capase 1 activity in the research laboratory

Caspase 1 Colorimetric Assay Kit

55R-1273 25 assays
EUR 277
Description: Assay Kit for detection of Capase 1 activity in the research laboratory

Caspase 8 Fluorometric Assay Kit

55R-1274 25 assays
EUR 277
Description: Assay Kit for detection of Capase 8 activity in the research laboratory

Caspase 8 Colorimetric Assay Kit

55R-1275 25 assays
EUR 277
Description: Assay Kit for detection of Capase 8 activity in the research laboratory

Caspase 6 Fluorometric Assay Kit

55R-1276 25 assays
EUR 287
Description: Assay Kit for detection of Capase 6 activity in the research laboratory

Caspase 6 Colorimetric Assay Kit

55R-1277 25 assays
EUR 284
Description: Assay Kit for detection of Capase 6 activity in the research laboratory

Caspase 2 Fluorometric Assay Kit

55R-1278 25 assays
EUR 284
Description: Assay Kit for detection of Capase 2 activity in the research laboratory

Caspase 2 Colorimetric Assay Kit

55R-1279 25 assays
EUR 303
Description: Assay Kit for detection of Capase 2 activity in the research laboratory

Caspase 9 Fluorometric Assay Kit

55R-1280 25 assays
EUR 277
Description: Assay Kit for detection of Capase 9 activity in the research laboratory

Caspase 9 Colorimetric Assay Kit

55R-1281 25 assays
EUR 296
Description: Assay Kit for detection of Capase 9 activity in the research laboratory

Caspase 5 Fluorometric Assay Kit

55R-1282 25 assays
EUR 284
Description: Assay Kit for detection of Capase 5 activity in the research laboratory

Caspase 5 Colorimetric Assay Kit

55R-1283 25 assays
EUR 303
Description: Assay Kit for detection of Capase 5 activity in the research laboratory

Caspase 10 Fluorometric Assay Kit

55R-1284 25 assays
EUR 284
Description: Assay Kit for detection of Capase 10 activity in the research laboratory

Caspase 10 Colorimetric Assay Kit

55R-1285 25 assays
EUR 303
Description: Assay Kit for detection of Capase 10 activity in the research laboratory

Caspase 4 Fluorometric Assay Kit

55R-1286 25 assays
EUR 303
Description: Assay Kit for detection of Capase 4 activity in the research laboratory

Caspase 4 Colorimetric Assay Kit

55R-1287 25 assays
EUR 312
Description: Assay Kit for detection of Capase 4 activity in the research laboratory

Caspase-1 Activity Assay Kit

AR4003-100Assays 100 Assays
EUR 514

Caspase-1 Activity Assay Kit

AR4003-25Assays 25 Assays
EUR 236

Caspase-1 Activity Assay Kit

AR4003-50Assays 50 Assays
EUR 340

Caspase-2 Activity Assay Kit

AR4004-100Assays 100 Assays
EUR 514

Caspase-2 Activity Assay Kit

AR4004-25Assays 25 Assays
EUR 236

Caspase-2 Activity Assay Kit

AR4004-50Assays 50 Assays
EUR 340

QuantiFluo Caspase-3 Assay Kit

DCS3-100 100
EUR 275
Description: Quantitative determination of the apoptosis target caspase-3 activity by fluorimetry (400/490nm) method. Procedure: 60 min. Kit size: 100 tests. Shelf life: 12 months.

Caspase 3/7 Assay Kit

20-abx299017
  • EUR 1302.00
  • EUR 582.00
  • 100 tests
  • 25 tests
  • Shipped within 1 week.

Caspase 3/7 Assay Kit

abx299024-2550tests 25-50 tests
EUR 692
  • Shipped within 1 week.

Caspase 3/7 Assay Kit

20-abx299028
  • EUR 1193.00
  • EUR 582.00
  • 100 tests
  • 25 tests
  • Shipped within 1 week.

Caspase 1 Assay Kit, green

KF17201 25 Tests
EUR 325

Caspase 2 Assay Kit, green

KF17202 25 Tests
EUR 304

Caspase 6 Assay Kit, green

KF17204 25 Tests
EUR 304

Caspase 8 Assay Kit, green

KF17205 25 Tests
EUR 304

Caspase 9 Assay Kit, green

KF17206 25 Tests
EUR 304

Caspase 10 Assay Kit, green

KF17207 25 Tests
EUR 304

Caspase 13 Assay Kit, green

KF17208 25 Tests
EUR 304

Caspase 9 Assay Kit, red

KF17302 25 Tests
EUR 304

660 Caspase-1 Assay Kit

KF17362 25-50 Tests
EUR 349

Caspase-5 Fluorometric Assay Kit

K2195-100 100 assays
EUR 502

Caspase-5 Fluorometric Assay Kit

K2195-200 200 assays
EUR 696

Caspase-5 Fluorometric Assay Kit

K2195-25 25 assays
EUR 238

Caspase-5 Fluorometric Assay Kit

K2195-400 400 assays
EUR 1114

Caspase-5 Colorimetric Assay Kit

K2196-100 100 assays
EUR 529

Caspase-5 Colorimetric Assay Kit

K2196-200 200 assays
EUR 725

Caspase-5 Colorimetric Assay Kit

K2196-25 25 assays
EUR 251

Caspase-5 Colorimetric Assay Kit

K2196-400 400 assays
EUR 1156

Caspase-10 Colorimetric Assay Kit

K2197-100 100 assays
EUR 529

Caspase-10 Colorimetric Assay Kit

K2197-200 200 assays
EUR 738

Caspase-10 Colorimetric Assay Kit

K2197-25 25 assays
EUR 251

Caspase-10 Colorimetric Assay Kit

K2197-400 400 assays
EUR 1114

Caspase-4 Fluorometric Assay Kit

K2198-100 100 assays
EUR 529

Caspase-4 Fluorometric Assay Kit

K2198-200 200 assays
EUR 738

Caspase-4 Fluorometric Assay Kit

K2198-25 25 assays
EUR 251

Caspase-4 Fluorometric Assay Kit

K2198-400 400 assays
EUR 1114

Caspase-4 Colorimetric Assay Kit

K2199-100 100 assays
EUR 557

Caspase-4 Colorimetric Assay Kit

K2199-200 200 assays
EUR 738

Caspase-4 Colorimetric Assay Kit

K2199-25 25 assays
EUR 266

Caspase-4 Colorimetric Assay Kit

K2199-400 400 assays
EUR 1114

Caspase-8 Fluorometric Assay Kit

K2012-100 100 assays
EUR 474

Caspase-8 Fluorometric Assay Kit

K2012-200 200 assays
EUR 696

Caspase-8 Fluorometric Assay Kit

K2012-25 25 assays
EUR 238

Caspase-8 Fluorometric Assay Kit

K2012-400 400 assays
EUR 1086

Caspase-8 Colorimetric Assay Kit

K2013-100 100 assays
EUR 474

Caspase-8 Colorimetric Assay Kit

K2013-200 200 assays
EUR 696

Caspase-8 Colorimetric Assay Kit

K2013-25 25 assays
EUR 238

Caspase-8 Colorimetric Assay Kit

K2013-400 400 assays
EUR 1086

Caspase-6 Fluorometric Assay Kit

K2014-100 100 assays
EUR 474

Caspase-6 Fluorometric Assay Kit

K2014-200 200 assays
EUR 696

Caspase-6 Fluorometric Assay Kit

K2014-25 25 assays
EUR 238

Caspase-6 Fluorometric Assay Kit

K2014-400 400 assays
EUR 1086

Caspase-6 Colorimetric Assay Kit

K2015-100 100 assays
EUR 487

Caspase-6 Colorimetric Assay Kit

K2015-200 200 assays
EUR 696

Caspase-6 Colorimetric Assay Kit

K2015-25 25 assays
EUR 238

Caspase-6 Colorimetric Assay Kit

K2015-400 400 assays
EUR 1101

Caspase-2 Fluorometric Assay Kit

K2016-100 100 assays
EUR 502

Caspase-2 Fluorometric Assay Kit

K2016-200 200 assays
EUR 696

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